Türkeş C.Söyüt H.Beydemir S.20.04.20192019-04-2020.04.20192019-04-2020161382-6689https://dx.doi.org/10.1016/j.etap.2015.11.024https://hdl.handle.net/20.500.12403/636In this study, we investigated the effects of the drugs, palonosetron hydrochloride, bevacizumab and cyclophosphamide, on human serum paraoxonase-I (hPON1) enzyme activity in in vitro conditions. The enzyme was purified ~231-fold with 34.2% yield by using ammonium sulphate precipitation, DEAE-Sephadex A-50 ion-exchange chromatography and Sephadex G-200 gel-filtration chromatography from human serum. hPON1 exhibited a single protein band on the SDS polyacrylamide gel electrophoresis. The inhibition studies were performed on paraoxonase activity of palonosetron hydrochloride, bevacizumab and cyclophosphamide. Ki constants were found as 0.033 ± 0.001, 0.054 ± 0.003 mM and 3.419 ± 0.518 mM, respectively. Compared to the inhibition rates of the drugs, palonosetron hydrochloride has the maximum inhibition rate. However, inhibition mechanisms of the drugs were determined as noncompetitive by Lineweaver-Burk curves. © 2016 Elsevier B.V.enginfo:eu-repo/semantics/closedAccessBevacizumabCyclophosphamideInhibitionPalonosetron hydrochlorideParaoxonasearyldialkylphosphatasearyldialkylphosphatase 1bevacizumabcyclophosphamidemessenger RNApalonosetronaryldialkylphosphatasebevacizumabcyclophosphamideDEAE-Sephadex A-50dextrandiethylaminoethyldextranenzyme inhibitorisoquinoline derivativepalonosetronquinuclidine derivativesephadexArticleblood levelcontrolled studydrug mechanismenzyme activityenzyme assayenzyme inhibitionenzyme purificationgel filtration chromatographygene expressionhumanIC50in vitro studyion exchange chromatographymolecular dynamicspolyacrylamide gel electrophoresispriority journalanalogs and derivativesmetabolismsize exclusion chromatographyAryldialkylphosphataseBevacizumabChromatography, GelCyclophosphamideDEAE-DextranDextransEnzyme InhibitorsHumansIsoquinolinesQuinuclidinesBevacizumabCyclophosphamideInhibitionPalonosetron hydrochlorideParaoxonasearyldialkylphosphatasearyldialkylphosphatase 1bevacizumabcyclophosphamidemessenger RNApalonosetronaryldialkylphosphatasebevacizumabcyclophosphamideDEAE-Sephadex A-50dextrandiethylaminoethyldextranenzyme inhibitorisoquinoline derivativepalonosetronquinuclidine derivativesephadexArticleblood levelcontrolled studydrug mechanismenzyme activityenzyme assayenzyme inhibitionenzyme purificationgel filtration chromatographygene expressionhumanIC50in vitro studyion exchange chromatographymolecular dynamicspolyacrylamide gel electrophoresispriority journalanalogs and derivativesmetabolismsize exclusion chromatographyAryldialkylphosphataseBevacizumabChromatography, GelCyclophosphamideDEAE-DextranDextransEnzyme InhibitorsHumansIsoquinolinesQuinuclidinesarticle42252257