Şahin E.Dertli E.20.04.20192019-04-2020.04.20192019-04-2020171612-1872https://dx.doi.org/10.1002/cbdv.201700121https://hdl.handle.net/20.500.12403/470The increasing demand for biocatalysts in synthesizing enantiomerically pure chiral alcohols results from the outstanding characteristics of biocatalysts in reaction, economic, and ecological issues. Herein, fifteen yeast strains belonging to three food originated yeast species Candida zeylanoides, Pichia fermentans, and Saccharomyces uvarum were tested for their capability for asymmetric reduction of acetophenone to 1-phenylethanol as biocatalysts. Of these strains, C. zeylanoides P1 showed an effective asymmetric reduction ability. Under optimized conditions, substituted acetophenones were converted to corresponding optically active secondary alcohols in up to 99% enantiomeric excess and at high yields. The preparative scale asymmetric bioreduction of 4-nitroacetophenone (1m) by C. zeylanoides P1 gave (S)-1-(4-nitrophenyl)ethanol (2m) with 89% yield and > 99% enantiomeric excess. Compound 2m has been obtained in an enantiomerically pure and inexpensive form. Additionally, these results indicate that C. zeylanoides P1 is a promising biocatalyst for the synthesis of chiral alcohols in industry. © 2017 Wiley-VHCA AG, Zurich, Switzerlandenginfo:eu-repo/semantics/closedAccessAsymmetric reductionBiocatalysisBiotransformationsCandida zeylanoidesWhole yeast cells4 nitroacetophenonealcohol derivative4-nitroacetophenoneacetophenoneacetophenone derivativebenzyl alcohol derivativemethylphenyl carbinolArticleasymmetric synthesisbiocatalysisbiocatalystbiotransformationCandida zeylanoideschiralityenantiomerenantioselectivityenzyme structurefermentationnonhumanorange juicePichiaPichia fermentansreduction (chemistry)Saccharomyces uvarumstereoselectivitywhole cellyeast cellCandidachemistryclassificationfood controlmetabolismmicrobiologyoxidation reduction reactionstereoisomerismAcetophenonesBenzyl AlcoholsBiocatalysisCandidaFood MicrobiologyIndustrial MicrobiologyOxidation-ReductionStereoisomerismAsymmetric reductionBiocatalysisBiotransformationsCandida zeylanoidesWhole yeast cells4 nitroacetophenonealcohol derivative4-nitroacetophenoneacetophenoneacetophenone derivativebenzyl alcohol derivativemethylphenyl carbinolArticleasymmetric synthesisbiocatalysisbiocatalystbiotransformationCandida zeylanoideschiralityenantiomerenantioselectivityenzyme structurefermentationnonhumanorange juicePichiaPichia fermentansreduction (chemistry)Saccharomyces uvarumstereoselectivitywhole cellyeast cellCandidachemistryclassificationfood controlmetabolismmicrobiologyoxidation reduction reactionstereoisomerismAcetophenonesBenzyl AlcoholsBiocatalysisCandidaFood MicrobiologyIndustrial MicrobiologyOxidation-ReductionStereoisomerismarticle149