Uzuncakmak, Sevgi KarabulutNaldan, Muhammet EminDirican, EbubekirKerget, FerhanHalici, Zekai2024-10-042024-10-0420220301-48511573-4978https://doi.org/10.1007/s11033-022-07710-9http://hdl.handle.net/20.500.12403/3614Background Coronavirus-19 is still considered a pandemic that influences the world. Other molecular alterations should be clearer besides the increasing cytokine storm and pro-inflammatory molecules. Hypoxic conditions that induce HIF-1 alpha lead to stimulate gene expression of STC-2 that targets PAPP-A expression. This study aimed to determine gene expression levels of PAPP-A, STC-2, and HIF-1 alpha in COVID-19 infection. We also aimed to reveal the relationship of these genes with laboratory and clinical data of COVID-19 patients. Materials and Results We extracted RNA from peripheral blood samples of COVID-19(+) and COVID-19(-) individuals. The real-time PCR method was used to measure mRNA expression of PAPP-A, STC-2, and HIF-1 alpha. Gene expression analysis was evaluated by the 2(-Delta Delta Ct) method. PAPP-A, STC-2, and HIF-1 alpha mRNA expressions of severe patients were higher than healthy individuals (p = 0.0451, p= 0.4466, p < 0.0001, respectively). Correlation analysis of gene expression patterns of severe patients demonstrated a positive correlation between PAPP-A and STC-2 (p <0.0001, r= 0.8638). Conclusion This is the first study that investigates the relation of PAPP-A, STC-2, and HIF-1 alpha gene expression in patients with COVID-19 infection. Besides the routine laboratory findings, PAPP-A, STC-2, and HIF-1 alpha mRNA expressions may be considered to patients' prognosis as a sign of increased cytokines and pro-inflammatory molecules.eninfo:eu-repo/semantics/openAccessSARS-CoV-2PAPP-ASTC-2HIF-1 alphaCOVID-19Article4998693869910.1007/s11033-022-07710-9357969372-s2.0-85133624726Q2WOS:000825011800002Q3