Türkeş C.Söyüt H.Beydemir Ş.20.04.20192019-04-2020.04.20192019-04-2020151475-6366https://dx.doi.org/10.3109/14756366.2014.959511https://hdl.handle.net/20.500.12403/684In this study, we investigated the effects of antibacterial drugs (moxifloxacin hydrochloride, levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodium) on human serum paraoxonase-1 (hPON1) enzyme activity from human serum in vitro conditions. For this purpose, hPON1 enzyme was purified from human serum using simple chromatographic methods. The antibacterial drugs exhibited inhibitory effects on hPON1 at low concentrations. K<inf>i</inf> constants were calculated to be 2.641 ± 0.040 mM, 5.525 ± 0.817 mM, 35.092 ± 1.093 mM, 252.762 ± 5.749 mM and 499.244 ± 10.149 mM, respectively. The inhibition mechanism of moxifloxacin hydrochloride was competitive, whereas levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodium were noncompetitive inhibitors. © 2014 Informa UK Ltd. All rights reserved.eninfo:eu-repo/semantics/closedAccessCefepime hydrochloridecefotaxime sodiumceftizoxime sodiuminhibitionlevofloxacin hemihidratemoxifloxacin hydrochlorideparaoxonasearyldialkylphosphatase 1cefepimecefotaximeceftizoximelevofloxacinmoxifloxacinaryldialkylphosphatasecefepimecefotaximeceftizoximecephalosporin derivativelevofloxacinmoxifloxacinPON1 protein, humanquinolone derivativeanion exchange chromatographyArticleenzyme activityenzyme inhibitiongel filtration chromatographyin vitro studyprecipitationpriority journalserumantagonists and inhibitorsbloodhumanAryldialkylphosphataseCefotaximeCeftizoximeCephalosporinsFluoroquinolonesHumansIn Vitro TechniquesLevofloxacinCefepime hydrochloridecefotaxime sodiumceftizoxime sodiuminhibitionlevofloxacin hemihidratemoxifloxacin hydrochlorideparaoxonasearyldialkylphosphatase 1cefepimecefotaximeceftizoximelevofloxacinmoxifloxacinaryldialkylphosphatasecefepimecefotaximeceftizoximecephalosporin derivativelevofloxacinmoxifloxacinPON1 protein, humanquinolone derivativeanion exchange chromatographyArticleenzyme activityenzyme inhibitiongel filtration chromatographyin vitro studyprecipitationpriority journalserumantagonists and inhibitorsbloodhumanAryldialkylphosphataseCefotaximeCeftizoximeCephalosporinsFluoroquinolonesHumansIn Vitro TechniquesLevofloxacinHuman serum paraoxonase-1 (hPON1): In vitro inhibition effects of moxifloxacin hydrochloride, levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodiumArticle30462262810.3109/14756366.2014.959511255197642-s2.0-84937199778Q1WOS:000359816600016Q1