Yılmaz D.Şahin E.Dertli E.20.04.20192019-04-2020.04.20192019-04-2020171612-1872https://dx.doi.org/10.1002/cbdv.201700269https://hdl.handle.net/20.500.12403/458Chiral secondary alcohols are valuable intermediates for many important enantiopure pharmaceuticals and biologically active molecules. In this work, we studied asymmetric reduction of aromatic ketones to produce the corresponding chiral secondary alcohols using lactic acid bacteria (LAB) as new biocatalysts. Seven LAB strains were screened for their ability to reduce acetophenones to their corresponding alcohols. Among these strains, Lactobacillus paracasei BD101 was found to be the most successful at reducing the ketones to the corresponding alcohols. The reaction conditions were further systematically optimized for this strain and high enantioselectivity (99%) and very good yields were obtained. These secondary alcohols were further tested for their antimicrobial activities against important pathogens and significant levels of antimicrobial activities were observed although these activities were altered depending on the secondary alcohols as well as their enantiomeric properties. The current methodology demonstrates a promising and alternative green approach for the synthesis of chiral secondary alcohols of biological importance in a cheap, mild, and environmentally useful process. © 2017 Wiley-VHCA AG, Zurich, Switzerlandeninfo:eu-repo/semantics/closedAccessAntibacterialBiocatalystChiral alcoholsEnantioselective bioreductionLactobacillus paracaseiacetophenone derivativealcohol derivativeketone derivativealcohol derivativeantiinfective agentalcohol productionantimicrobial activityArticleasymmetric catalysisBacillus cereusbacterial strainbiocatalystchiralityconcentration (parameters)enantiomerenantioselectivityEnterococcus faeciumEscherichia colievaluation studylactic acid bacteriumLactobacillus fermentumLactobacillus paracaseiLactobacillus plantarumnonhumannucleotide sequencequantum yieldreduction (chemistry)Salmonella enterica serovar TyphimuriumStaphylococcus aureustemperature sensitivityYersinia enterocoliticabiocatalysischemistrydrug effectsenzyme specificityGram negative bacteriumGram positive bacteriumLactobacillus paracaseimetabolismmicrobial sensitivity testoxidation reduction reactionpHstereoisomerismtemperaturetime factorAcetophenonesAlcoholsAnti-Infective AgentsBiocatalysisGram-Negative BacteriaGram-Positive BacteriaHydrogen-Ion ConcentrationLactobacillus paracaseiMicrobial Sensitivity TestsOxidation-ReductionStereoisomerismSubstrate SpecificityTemperatureTime FactorsAntibacterialBiocatalystChiral alcoholsEnantioselective bioreductionLactobacillus paracaseiacetophenone derivativealcohol derivativeketone derivativealcohol derivativeantiinfective agentalcohol productionantimicrobial activityArticleasymmetric catalysisBacillus cereusbacterial strainbiocatalystchiralityconcentration (parameters)enantiomerenantioselectivityEnterococcus faeciumEscherichia colievaluation studylactic acid bacteriumLactobacillus fermentumLactobacillus paracaseiLactobacillus plantarumnonhumannucleotide sequencequantum yieldreduction (chemistry)Salmonella enterica serovar TyphimuriumStaphylococcus aureustemperature sensitivityYersinia enterocoliticabiocatalysischemistrydrug effectsenzyme specificityGram negative bacteriumGram positive bacteriumLactobacillus paracaseimetabolismmicrobial sensitivity testoxidation reduction reactionpHstereoisomerismtemperaturetime factorAcetophenonesAlcoholsAnti-Infective AgentsBiocatalysisGram-Negative BacteriaGram-Positive BacteriaHydrogen-Ion ConcentrationLactobacillus paracaseiMicrobial Sensitivity TestsOxidation-ReductionStereoisomerismSubstrate SpecificityTemperatureTime FactorsArticle141110.1002/cbdv.201700269287926672-s2.0-85031725613Q2WOS:000416313000010Q3