Ispirli, HumeyraYuzer, Mustafa OnurSkory, ChristopherColquhoun, Ian J.Sagdic, OsmanDertli, Enes2024-10-042024-10-0420191024-24221029-2446https://doi.org/10.1080/10242422.2019.1593969http://hdl.handle.net/20.500.12403/3076Glucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of alpha-glucans with different structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified and heterologously expressed. Alignments of GTFA with other glucansucrases revealed its novelty and a putative 3D model structure was obtained. The biochemical properties of the truncated enzyme without the N-terminal variable region, GTFA-Delta N, was characterized. The K-m and V-max were found to be 7.5 mM and 1.49 IU/mg, respectively, and it showed optimum activities at pH 7 and at 50 degrees C. The GTFA-Delta N produced in vitro an alpha-glucan with (alpha 1 -> 3) and (alpha 1 -> 6) glycosidic linkages using sucrose as the substrate. Importantly, GTFA-Delta N synthesized DP = 9 oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively, as detected by TLC, HPLC, LC-MS and NMR analysis.eninfo:eu-repo/semantics/closedAccessGlucansucraseglucanmalto-oligosaccharidesArticle37642143010.1080/10242422.2019.15939692-s2.0-85065527836Q3WOS:000469706400001Q3