Söyüt H.Kaya E.D.Beydemir S.20.04.20192019-04-2020.04.20192019-04-2020142221-1691https://dx.doi.org/10.12980/APJTB.4.2014APJTB-2014-0059https://hdl.handle.net/20.500.12403/809Objective: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). Methods: hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. Results: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. Conclusions: Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro. © 2014 by the Asian Pacific Journal of Tropical Biomedicine.eninfo:eu-repo/semantics/openAccessCefoperazone sodiumInhibitionMeropenem trihydrateParaoxonasePiperacillin sodiumaryldialkylphosphatase 1cefoperazonemeropenempiperacillinanion exchange chromatographyArticlebinding affinitycompetitive inhibitionconcentration (parameters)controlled studydrug protein bindingenzyme activityenzyme inhibitionenzyme purificationgel filtration chromatographyhumanIC50in vitro studyCefoperazone sodiumInhibitionMeropenem trihydrateParaoxonasePiperacillin sodiumaryldialkylphosphatase 1cefoperazonemeropenempiperacillinanion exchange chromatographyArticlebinding affinitycompetitive inhibitionconcentration (parameters)controlled studydrug protein bindingenzyme activityenzyme inhibitionenzyme purificationgel filtration chromatographyhumanIC50in vitro studyArticle4860360910.12980/APJTB.4.2014APJTB-2014-00592-s2.0-84922624356N/A