Ispirli, HumeyraBowman, Michael J.Skory, Christopher D.Dertli, Enes2024-10-042024-10-0420212212-42922212-4306https://doi.org/10.1016/j.fbio.2021.101388http://hdl.handle.net/20.500.12403/3719Cellobiose-derived oligosaccharides were produced from acceptor reactions with glucansucrase E81 and their prebiotic potentials were determined in this study. The major product was a mixture of trisaccharides formed by the addition of an alpha-linked glucose to cellobiose at either the 2-O position of the reducing end (86%) glucose or the 6-O position of the non-reducing end glucose (11%) demonstrated by NMR spectroscopy and LC-MS-LTQ analysis. The purified DP 3 cellobiose-derived oligosaccharides were then tested for in vitro human gastric juice and alpha-amylase digestion and no digestion was observed during the 6 h digestion period. Importantly, DP 3 cellobiose-derived oligosaccharides promoted the growth of Bifidobacteria at high levels demonstrating a Bifidogenic effect and a mild level of proliferation was observed for Lactobacilli suggesting the prebiotic roles of cellobiose-derived oligosaccharides.eninfo:eu-repo/semantics/openAccessGlucansucrasePrebiotic oligosaccharidesCellobiose-derived oligosaccharidesArticle4410.1016/j.fbio.2021.1013882-s2.0-85116322339Q1WOS:000710483900002Q1