Ispirli, HumeyraColquhoun, Ian J.Sahin, EnginSagdic, OsmanDertli, Enes2024-10-042024-10-0420190008-62151873-426Xhttps://doi.org/10.1016/j.carres.2019.107837http://hdl.handle.net/20.500.12403/3616Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively. The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis. The glycosylation of the gentiobiose with alpha-(1 -> 6) linkages and alpha-(1 -> 3) linkages was shown by H-1 and C-13 NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides. The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains. Gentiobiosederived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-alpha cytokines in HT29 cells in a dose dependent manner. This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.eninfo:eu-repo/semantics/closedAccessGlucansucraseGentiobiose-derived oligosaccharidesImmune modulationPrebioticArticle48610.1016/j.carres.2019.107837316554182-s2.0-85073676607Q2WOS:000496174300018Q2