Şahin, EnginSerencam, HüseyinDertli, Enes2020-11-252020-11-252019Şahin, E., Serencam, H., & Dertli, E. (2019). Production of enantiomerically pure (S)-phenyl (pyridin-2-yl) methanol with Lactobacillus paracasei BD101. Biocatalysis and Biotransformation, 37(6), 448-454.https://www.tandfonline.com/doi/full/10.1080/10242422.2019.1602611?casa_token=jJmNDvcqM3MAAAAA%3AJiXv5YrAm94IfC1WII6vfDLBgu6cp1_wLquy0yeHDGaLQyaqFAuK0N8_ONDWrxWPVPFYkv0hiKmDcZ0https://hdl.handle.net/20.500.12403/2230Asymmetric reduction studies of heteroaryl ketones, including phenyl(pyridin-2-yl)methanone in enantioselective form with biocatalysts are very few, and chiral heteroaryl alcohols have been synthesized generally in the small scale. In this study, seven bacterial strains have been used to produce the (S)-phenyl(pyridin-2-yl)methanol in high enantiomeric excess and yield. Among the tested strains, Lactobacillus paracasei BD101, was found to be the best biocatalyst for the reducing phenyl(pyridin-2-yl)methanone to the (S)-phenyl(pyridin-2-yl)methanol at gram scale. The asymmetric bioreduction conditions were systematically optimized using L. paracasei BD101, which demonstrated excellent enantioselectivity and high level of conversion for the bioreduction reaction. (S)-phenyl(pyridin-2-yl)methanol, which is an analgesic, was produced enantiomerically pure form in the first time on gram scale using a biocatalyst. In total, 5.857 g of (S)-phenyl(pyridin-2-yl)methanol in enantiomerically pure form (>99% enantiomeric excess) was obtained in 52 h with 93% yield using whole cells of L. paracasei BD101. Enantiomerically pure (S)-phenyl (pyridin-2-yl)methanol, which is an analgesic, was first produced in the gram scale using a biocatalyst with excellent ee (>99%) and yield (93%).enChirality, enantioselectivity, biocatalyst, L. paracasei BD101, (S)-phenyl(pyridin-2-yl)methanolArticle37644845410242422.2019.16026112-s2.0-85065652258Q3WOS:000470500000001Q3