Alim Z.Kilinc N.Sengul B.Beydemir S.20.04.20192019-04-2020.04.20192019-04-2020171095-6670https://dx.doi.org/10.1002/jbt.21898https://hdl.handle.net/20.500.12403/489Aldose reductase (AR) inhibitors play a vital importance as a potential therapeutic and preventive medicine when it comes to hyperglycemia associated diabetic complications. Additionally, capsaicin is used as a food additive and a drug in a number of diverse clinical trials. The aim of this study is to determine the in vitro inhibition behavior of capsaicin on AR enzyme activity, which was obtained from different rat tissues (heart, kidney, liver, and brain). We showed that AR was inhibited by capsaicin in the micromolar range and noncompetitive manner in all of the tissues. Ki values of capsaicin were found to be 8.87, 264, 535, and 597, respectively, in heart AR, kidney AR, liver AR, and brain AR. In conclusion, capsaicin may be an effective molecule when used in low concentrations to prevent diabetic complications associated with the polyol pathway. © 2017 Wiley Periodicals, Inc.eninfo:eu-repo/semantics/closedAccessaldose reductasecapsaicindiabetic complicationsinhibitionaldehyde reductasecapsaicinaldehyde reductasecapsaicinenzyme inhibitoranimal tissueArticlebraincontrolled studydrug efficacydrug mechanismenzyme activityenzyme inhibitionheartin vitro studykidneylivermalenonhumanratanimalantagonists and inhibitorsantibody specificitydose responsemetabolismSprague Dawley ratAldehyde ReductaseAnimalsCapsaicinDose-Response Relationship, DrugEnzyme InhibitorsMaleOrgan SpecificityRatsRats, Sprague-Dawleyaldose reductasecapsaicindiabetic complicationsinhibitionaldehyde reductasecapsaicinaldehyde reductasecapsaicinenzyme inhibitoranimal tissueArticlebraincontrolled studydrug efficacydrug mechanismenzyme activityenzyme inhibitionheartin vitro studykidneylivermalenonhumanratanimalantagonists and inhibitorsantibody specificitydose responsemetabolismSprague Dawley ratAldehyde ReductaseAnimalsCapsaicinDose-Response Relationship, DrugEnzyme InhibitorsMaleOrgan SpecificityRatsRats, Sprague-DawleyMechanism of capsaicin inhibition of aldose reductase activityArticle31710.1002/jbt.21898282179472-s2.0-85013411153Q2WOS:000405321900004Q4