Ozdemir, AkinSahin, Engin2024-10-042024-10-0420220899-00421520-636Xhttps://doi.org/10.1002/chir.23430http://hdl.handle.net/20.500.12403/3547Aromatic alpha-halohydrins, especially 2-haloethanols, which are a common pharmacological precursor, may be readily transformed to chiral beta-adrenergic receptor blockers. Studies including the synthesis of (S)-2-bromo-1-(naphthalen-2-yl)ethanol ((S)-2), an alpha-halohydrin, in high conversion, enantiomeric excess (ee), and yield by biocatalytic asymmetric reduction of 2-bromo-1-(naphthalen-2-yl)ethanone (1) are still insufficient. Moreover, asymmetric reduction of substrate 1 using a mathematical optimization method is not explored in the current literature. In this article, the four asymmetric bioreduction conditions, which are (1) pH, (2) temperature, (3) incubation period, and (4) agitation speed, of substrate 1 were optimized to obtain (S)-2 with A-optimal design-embedded model in the presence of Enterococcus faecium BY48. Optimum bioreduction conditions were determined by the A-optimal design-embedded model as follows: pH = 7, temperature = 25 degrees C, incubation period = 24 h, and agitation speed = 200 rpm. And then, it was suggested that (S)-2 could be obtained with 98.88% ee and 100% conversion rate (cr) under these optimum conditions. As a result of the experimental reaction performed under the optimization conditions suggested by the model, (S)-2 was obtained with 99% ee and 100% cr. The study revealed that E. faecium BY48 could be used as a biocatalyst in asymmetric reduction reactions. Also, the A-optimal design-embedded model could have the great potential to obtain the optimum asymmetric bioreduction conditions.eninfo:eu-repo/semantics/closedAccess(S)-2-bromo-1-(naphthalen-2-yl)ethanolA-optimal designasymmetric bioreductionchiral halohydrinsoptimizationwhole-cell biocatalystArticle34579680610.1002/chir.23430352180762-s2.0-85125241769Q3WOS:000761178000001Q3