Alim Z.Kilinç N.Şengül B.Beydemir Ş.20.04.20192019-04-2020.04.20192019-04-2020171475-6366https://dx.doi.org/10.1080/14756366.2016.1250752https://hdl.handle.net/20.500.12403/567Aldose reductase (AR) inhibitors have vital importance in the treatment and prevention of diabetic complications. In this study, rat kidney AR was purified 19.34-fold with a yield of 3.49% and a specific activity of 0.88 U/mg using DE-52 Cellulose anion exchange chromatography, gel filtration chromatography and 2?5? ADP Sepharose-4B affinity chromatography, respectively. After purification, the in vitro inhibition effects of some phenolic acids (tannic acid, chlorogenic acid, sinapic acid, protocatechuic acid, 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillic acid, syringic acid, ?-resorcylic acid, 3-hydroxybenzoic acid and gallic acid) were investigated on purified enzyme. We determined IC50, Ki values and inhibition types of these phenolic acids. As a result, tannic and chlorogenic acid had a strong inhibition effect. On the other hand, gallic acid had a weak inhibition effect. In this study, all phenolic acids except for chlorogenic acid and p-coumaric acid showed non-competitive inhibition effects on rat kidney AR. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.eninfo:eu-repo/semantics/openAccessAldose reductasediabetic complicationsinhibitionphenolic acids3 hydroxybenzoic acid4 hydroxybenzoic acidadenosine diphosphatealdehyde reductasealpha resorcylic acidcellulosechlorogenic acidde 52 celluloseferulic acidgallic acidpara coumaric acidphenol derivativeprotocatechuic acidsepharosesinapic acidsyringic acidtanninunclassified drugvanillic acidaldehyde reductasehydroxybenzoic acid derivativephenolic acidaffinity chromatographyanion exchange chromatographyantioxidant activityArticledrug inhibitionenzyme activityenzyme purificationgel filtration chromatographyIC50in vitro studykidneynonhumanpriority journalprotein foldingratanimalantagonists and inhibitorsenzymologykidneypolyacrylamide gel electrophoresisAldehyde ReductaseAnimalsElectrophoresis, Polyacrylamide GelHydroxybenzoatesIn Vitro TechniquesKidneyRatsAldose reductasediabetic complicationsinhibitionphenolic acids3 hydroxybenzoic acid4 hydroxybenzoic acidadenosine diphosphatealdehyde reductasealpha resorcylic acidcellulosechlorogenic acidde 52 celluloseferulic acidgallic acidpara coumaric acidphenol derivativeprotocatechuic acidsepharosesinapic acidsyringic acidtanninunclassified drugvanillic acidaldehyde reductasehydroxybenzoic acid derivativephenolic acidaffinity chromatographyanion exchange chromatographyantioxidant activityArticledrug inhibitionenzyme activityenzyme purificationgel filtration chromatographyIC50in vitro studykidneynonhumanpriority journalprotein foldingratanimalantagonists and inhibitorsenzymologykidneypolyacrylamide gel electrophoresisAldehyde ReductaseAnimalsElectrophoresis, Polyacrylamide GelHydroxybenzoatesIn Vitro TechniquesKidneyRatsArticle32127728410.1080/14756366.2016.1250752281119962-s2.0-85013846384Q1WOS:000392591100021Q1