Kizil, Hamit Emre2026-02-282026-02-2820250250-5371https://doi.org/10.18805/LRF-871https://hdl.handle.net/20.500.12403/6146Background: Silver nanoparticles (AgNPs) have gained significant attention due to their unique antimicrobial, catalytic and optical properties. This study focuses on the green synthesis of silver nanoparticles using black rosehip (Rosa pimpinellifolia L.) seeds (Rp-AgNPs) and evaluates their biological activities. Methods: Rp-AgNPs were synthesized using R. pimpinellifolia seed extract and characterized using multiple analytical techniques including UV-Vis spectroscopy, XRD, FTIR, TEM, SEM, EDAX, zeta potential analysis, DSC, SERS and XPS. The biological activities were assessed through DPPH free radical scavenging assay, cytotoxicity testing against H460 lung cancer cells and antimicrobial activity evaluation against various pathogens. Result: The synthesized Rp-AgNPs exhibited spherical morphology with an average size of 51.27 nm and demonstrated stability with a zeta potential of-18.9. The nanoparticles showed enhanced antioxidant activity (IC50 = 30.89 mu g/mL) compared to the seed extract (IC50 = 136.4 mu g/mL). Cytotoxicity studies revealed significant effects against H460 cells with an IC50 of 65.8 mu g/mL. Antimicrobial testing demonstrated effectiveness against both Gram-positive and Gram-negative bacteria (MIC 50-100 mu g/mL) and Candida strains (MIC 100-200 mu g/mL).eninfo:eu-repo/semantics/openAccessBiological activityBlack rosehip (Rosa pimpinellifolia L.)Green synthesisSilver nanoparticlesArticle4891487149610.18805/LRF-8712-s2.0-105020722455Q3WOS:001570892900010Q3