Ozdemir, M.Sonmez, Z.Aksakal, V.2024-10-042024-10-0420211018-70812309-8694https://doi.org/10.36899/JAPS.2021.3.0279http://hdl.handle.net/20.500.12403/3025The aim of this study was to ascertain the Rsal polymorphism of the Prolactin (PRL) gene of 186 organically reared Holstein cattle by PCR-RFLP method, in order to reveal the genetic variation of the population, and to reveal the relationship between PRL/RsaI genotypes and some milk yield traits. In the study, the RsaI(-/-) genotype frequency, RsaI(+/+) genotype frequency and RsaI(-/+) genotype frequency of Holstein cattle were determined as 0.26, 0.22 and 0.52, respectively. The Rsal(-) gene frequency was 0.52, and the RsaI(+) gene frequency was 0.48. Hardy-Weinberg genetic equilibrium test showed that the distribution of genotype frequencies was determined to be in genetic equilibrium. The relationships between PRL/Rsal polymorphism and the examined milk yield traits were not significant. As a result, an economic and safe method PCR-RFLP for the detection of genetic markers, was successfully used in the determination of the 3 genotypes (RsaI(-/-), RsaI(-/+), and RsaI(+/+)) of Prolactin gene in Holstein cattle reared under organic condition, and the results was similar with previously reported literature.eninfo:eu-repo/semantics/openAccessProlactinPCR-RFLPpolymorphismmarkercattleArticle31390090510.36899/JAPS.2021.3.02792-s2.0-85100173553Q4WOS:000697050800029Q4