Sahin, Engin2024-10-042024-10-0420232468-8231https://doi.org/10.1016/j.mcat.2023.113037http://hdl.handle.net/20.500.12403/3255Asymmetric bioreduction catalyzed by biocatalyst have shown considerable potential in the preparation of chiral alcohols. (R)-1-(furan-2-yl)ethanol ((R)-2) can be used precursor in the synthesis of many naturally bioactive piperidine alkaloids such as (-)-Cassine, (-)-Spectaline, (-)-Carnavaline, Prosafrine and (-)-Prosafrinine. Moreover, (R)-2 a key chiral precursor in the production of alpha, beta-unsaturated delta-lactones, which have antifungal, antibiotic, and cytotoxic effects on human tumor cells. However, the synthesis routes of (R)-2 still pose signif-icant challenges in term of unsatisfactory enantiomeric excess (ee) and gram scale synthesis. In this study, Lactobacillus paracasei BD101 biocatalyst from boza, a cereal-based fermented beverage, for the asymmetric bioreduction of the 1-(furan-2-yl)ethanone (1). Following biocatalytic process optimization, (R)-2 was generated with >99% ee and 97% yield. In addition, 9.9 g of 1 was completely converted into (R)-2 on a gram scale (9.78 g, 97% isolated yield) in 48 h. This is the first report about the fabrication of enantiopure (R)-2 in high gram scale using biocatalyst. The optical purity of (R)-2 produced by asymmetric reduction with L paracasei BD101 was also noteworthy since it was the highest documented to date. This study provides an efficient green process for the biocatalytic synthesis of (R)-2.eninfo:eu-repo/semantics/closedAccessDrug precursorWhole-cell biocatalystAsymmetric bioreductionChiral secondary alcohol(R)-1-(furan-2-yl)ethanolArticle53910.1016/j.mcat.2023.1130372-s2.0-85149213014Q2WOS:000949059000001Q2