Characterization of a 4,6???glucanotransferase from Lactobacillus reuteri E81 and production of malto-oligosaccharides with immune-modulatory roles
Abstract
A wide number of Lactic Acid Bacteria (LAB) species produce ?-glucans with their ability to synthesize glucansucrases (GS) which use sucrose as substrate for the glucan production. Recently another group of enzymes in LAB gained special interest for their ability to produce ?-glucans targeting the substrates containing ?1-4-linkages and synthesizing new (?1-6) or (?1-3)–linkages as ??glucanotransferases. In this study, a putative 4,6???glucanotransferase (GTFB) from sourdough isolate Lactobacillus reuteri E81 was identified and expressed in Escherichia coli. The biochemical characterization of the GTFB-E81 confirmed its function as it cleaved the ?1-4-linkages in different substrates and produced new gluco-oligomers/polymers containing ?1-6 linkages together with the ?1-4-linkages detected by NMR analysis. GTFB-E81 produced malto-oligosaccharides targeting maltose and maltoheptaose as substrates with up to DP 8 detected by TLC and ESI-MS/MS analysis. The functional roles of these malto-oligosaccharides were determined by testing their immune-modulatory functions in HT29 cells and they triggered the production of anti-inflammatory 1L-4 and pro-inflammatory IL-12 cytokines. © 2018 Elsevier B.V.
Source
International Journal of Biological MacromoleculesVolume
124Collections
Related items
Showing items related by title, author, creator and subject.
-
Structural analysis of the ?-D-glucan produced by the sourdough isolate Lactobacillus brevis E25
Dertli E.; Colquhoun I.J.; Côté G.L.; Le Gall G.; Narbad A. (Elsevier Ltd, 2018)Cereal-associated Lactic Acid Bacteria (LAB) are well known for homopolymeric exopolysaccharide (EPS) production. Herein, the structure of an EPS isolated from sourdough isolate Lactobacillus brevis E25 was determined. A ... -
Physicochemical characterisation of an ?-glucan from Lactobacillus reuteri E81 as a potential exopolysaccharide suitable for food applications
İspirli H.; Sagdic O.; Yılmaz M.T.; Dertli E. (Elsevier Ltd, 2019)Lactobacillus reuteri strain (E81) is an alpha glucan producer from traditional sourdough and the physicochemical characteristics of this glucan was determined in this study. The HPLC analysis confirmed the presence of ... -
Spontaneous Mutation Reveals Influence of Exopolysaccharide on Lactobacillus johnsonii Surface Characteristics
Horn N.; Wegmann U.; Dertli E.; Mulholland F.; Collins S.R.A.; Waldron K.W.; Bongaerts R.J.; Mayer M.J.; Narbad A. (2013)As a competitive exclusion agent, Lactobacillus johnsonii FI9785 has been shown to prevent the colonization of selected pathogenic bacteria from the chicken gastrointestinal tract. During growth of the bacterium a rare but ...