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    Microbial Production of Galactooligosaccharides
    (Springer Science+Business Media, 2025) Durgut-Malçok, Senanur; Oztekin, Sebahat; Adal, Samiye; İnan-Çınkır, Nuray; Çelebi, Yasemin; Çalışkan Koç, Gülşah; Delikanli-Kiyak, Berrak
    Galactooligosaccharides (GOSs) are prebiotic compounds derived from lactose, known for their ability to selectively promote the growth of beneficial gut microbiota, like bifidobacteria and lactobacilli. Their physiological benefits include enhanced gut health, improved immune function, and increased mineral absorption. Produced primarily through enzymatic synthesis using microbial ?-galactosidases, GOSs offer an efficient and environmentally friendly alternative to chemical production methods. Recent advancements in enzyme immobilization and biotechnological approaches have optimized GOS yields, enabling their incorporation into a variety of food and health applications, including infant formulas, dairy products, and clinical supplements. The structural diversity of GOSs, characterized by their glycosidic linkages and degrees of polymerization, contributes to their functional versatility and prebiotic efficacy. Furthermore, their stability under acidic and high-temperature conditions makes them suitable for industrial processes. Despite these advancements, challenges such as cost reduction, process optimization, and the use of alternative substrates persist. Future research on enzyme engineering and tailored GOS formulations is anticipated to address these issues, expanding their applications in functional foods, nutraceuticals, and personalized health solutions. GOSs remain a promising tool for improving health and meeting diverse nutritional needs. © 2025 Springer Nature Switzerland AG.
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    Thawing frozen foods: A comparative review of traditional and innovative methods
    (Wiley, 2025) Koc, Gulsah Caliskan; Karabacak, Azime Ozkan; Sufer, Ozge; Adal, Samiye; Celebi, Yasemin; Kiyak, Berrak Delikanli; Oztekin, Sebahat
    Due to the changing consumer lifestyles, the tendency to adopt foods that require less preparation time and offer both variety and convenience has played a significant role in the development of the frozen food industry. Freezing is one of the fundamental food preservation techniques, as it maintains high product quality. Freezing reduces chemical and enzymatic reactions, lowers water activity, and prevents microbial growth, thereby extending the shelf life of foods. The freezing and thawing procedures directly impact the quality of frozen foods. The degree of tissue damage is determined by the freezing rate and the structure of the ice crystals that form during the freezing process. Generally, thawing occurs more slowly than freezing. During thawing, microorganisms, as well as chemical and physical changes, can cause nutrient damage. Thus, the goal of this review is to identify innovative and optimal thawing strategies. In order to save energy and/or improve quality, new chemical and physical thawing aids are being developed alongside emerging techniques such as microwave-assisted, ohmic-assisted, high pressure, acoustic thawing, and so on. In addition to discussing the possible uses of these technologies for the thawing process and their effects on food quality, the purpose of this study is to present a thorough comparative overview of recent advancements in thawing techniques.