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Öğe An Integrated In Vitro and In Silico Investigation of the Bioactive Properties of Wild Glycyrrhiza glabra var. glandulifera(Springer, 2025) Kizil, Hamit Emre; Ulcay, Sibel; Ekincioglu, Yavuz; Ogutcu, Hatice; Agar, GulerayThis investigation examined methanol extracts obtained from the whole plant (encompassing all organs except roots), flowers, and fruits of wild Glycyrrhiza glabra var. glandulifera, a liquorice cultivar indigenous to the Cappadocia region of T & uuml;rkiye, constituting the first comprehensive in vitro and in silico analysis of this species within the region. Anatomical identification revealed distinctive structural characteristics, including thick-walled epidermal cells in the stem, organized clusters of collenchyma cells, and well-defined sclerenchyma layers encompassing the vascular bundles. The petiole exhibited a characteristic heart-shaped morphology containing three vascular bundles, while foliar analysis demonstrated the presence of stomata on both adaxial and abaxial surfaces, accompanied by a bifacial mesophyll arrangement. The extracts demonstrated remarkable antimicrobial efficacy against both Gram-positive and Gram-negative bacteria, as well as various fungal species. Furthermore, significant cytotoxic activity was observed against non-small cell lung cancer (H460), with the whole plant extract exhibiting IC50 values of 117.8 mu g/mL (24 h) and 105.6 mu g/mL (48 h). Flower extracts demonstrated IC50 values of 116.8 mu g/mL (24 h) and 112.7 mu g/mL (48 h), while fruit extracts displayed superior potency with IC50 values of 104.4 mu g/mL (24 h) and 63.09 mu g/mL (48 h). Molecular docking analyses, conducted utilizing AutoDock Tools 1.5.6 and BIOVIA Discovery Studio Visualizer 4.5, revealed significant protein-ligand interactions, yielding optimal binding energy and inhibition constant values of -9.57 kcal/mol and 96.92 nM, respectively. The RMSD values were calculated based on structural variations between ligand-protein complexes during re-docking procedures. These findings underscore the substantial biological potential of Glycyrrhiza glabra var. glandulifera from Cappadocia, necessitating further comprehensive investigation.Öğe Anti-genotoxic Effects of Schiff bases and their Mn(III) Complexes Containing L-Aspartic acid and L-Phenylalanine(Gazi Univ, 2018) Hasanoglu Ozkan, Elvan; Kizil, Hamit Emre; Sakiyan, Iffet; Nartop, Dilek; Sari, Nursen; Agar, GulerayThe purpose of the research was to evaluate the genotoxic and anti-genotoxic properties of Schiff bases and their Mn (III) complexes containing L-aspartic acid, and L-phenylalanine. The anti-genotoxic properties of four compounds in human lymphocytes cells were investigated by sister chromatid exchanges (SCEs) test system against aflatoxin Bi (AFBi). The results showed that compounds have strong anti-genotoxic properties.Öğe Antiproliferative and apoptotic properties of vulpinic acid on A549, AGS and HeLa cells(Elsevier Science Bv, 2017) Kizil, Hamit Emre; Agar, Guleray[Abstract Not Available]Öğe Antiproliferative effects of Evernic acid on A549 and healthy human cells: An in vitro study(Elsevier Science Bv, 2015) Kizil, Hamit Emre; Agar, Guleray; Anar, Mustafa[Abstract Not Available]Öğe Cytotoxic and antiproliferative effects of evernic acid on HeLa cell lines: A candidate anticancer drug(Elsevier Science Bv, 2014) Kizil, Hamit Emre; Agar, Guleray; Anar, Mustafa[Abstract Not Available]Öğe DETERMINATION OF ANTIGENOTOXIC EFFECTS OF FOUR LICHEN SPECIES BY USING HUMAN LYMPHOCYTES(Parlar Scientific Publications (P S P), 2015) Anar, Mustafa; Aslan, Ali; Ceker, Selcuk; Kizil, Hamit Emre; Alpsoy, Lokman; Agar, GulerayIn this study, the geno-toxic and antigeno-toxic properties of the total extracts of four lichen species were investigated by using human lymphocytes. We obtained total extracts from R. farinacea, X parietina, U articulata and U. filipendula lichen species. The results of our studies showed that 5 mu M concentrations of AFB(1) changed the frequencies of micronucleus (MN) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GPx) activities. When 5, 10 and 20 mu g/ml concentrations of total extract were added to AFB(1), the frequencies of MN and MDA levels were decreased, and SOD, GSH and GPx levels were increased. Consequently, our findings show that four lichen total extracts have strong antigenotoxic properties against aflatoxin B-1.Öğe The determination of antioxidative effect and anticancer potential of vulpinic acid(Elsevier Science Bv, 2014) Anar, Mustafa; Kizil, Hamit Emre; Agar, Guleray[Abstract Not Available]Öğe Determination of Cytotoxic and Apoptotic Properties of Lobaric Acid, a Secondary Metabolite of Lichen and Investigation of Its Theoretical Potential(2024) Kızıl, Hamit Emre; Agar, Guleray; Ekincioğlu, YavuzIn this study, we aimed to elucidate some of the mechanisms of cell death induced by lobaric acid in A549 (human lung cancer) cells. For this purpose, the effects of cytotoxic concentrations on p53 and caspase-3 gene expressions were investigated. A549 cells were treated with varying concentrations of lobaric acid (12.5, 25, 50, and 100 µg/ml) for 48 hours and then their viability was evaluated and p53 and caspase-3 mRNA expressions were determined at statistically cytotoxic concentrations of 12.5, 50, and 100 µg/ml. According to beta-actin, it was determined that the increase in lobaric acid concentration revealed an upward trend in p53 and caspase-3 mRNA expressions. Furthermore, quantum chemical parameters such as frontier molecular orbitals, band gap energy and ionization potential, electronic affinity, chemical softness, chemical potential, electrophilicity index and chemical hardness were analyzed. Furthermore, molecular docking was performed to identify the binding sites and the binding behavior of lobaric acid to some target proteins (P53, Caspase-3 and Bcl-2).Öğe Determination of cytotoxic and proliferative effects of 2-amino-5-nitrobenzonitrile on AGS cancer cell line(Elsevier Science Bv, 2015) Anar, Mustafa; Kizil, Hamit Emre; Agar, Guleray[Abstract Not Available]Öğe Determination of the Antigenotoxic effects of Evernic acid against genotoxic effects of CCL4 on HUVEC lines(Elsevier Science Bv, 2017) Agar, Guleray; Kizil, Hamit Emre; Ceker, Selcuk; Capik, Ozel; Sengul, Bulent[Abstract Not Available]Öğe Pharmacological Potential and Bioactive Components of Wild Anatolian Sage (Salvia aethiopis L.)(Wiley, 2025) Bilginoglu, Emine; Kizil, Hamit Emre; Ogutcu, Hatice; Agar, Guleray; Bagci, YavuzSalvia aethiopis L., a member of the Lamiaceae family, has gained attention due to its bioactive compounds with potential therapeutic applications. This study investigated the chemical composition and biological activities of S. aethiopis ethanol extract collected from Karaman province, T & uuml;rkiye. The phenolic profile of the plant was characterized using LC-MS/MS analysis, which revealed substantial amounts of rosmarinic acid, ferulic acid, caffeic acid, and p-coumaric acid. The antimicrobial activity of the extracts was evaluated against various Gram-positive and Gram-negative bacteria, as well as fungal strains, using the agar well diffusion method. The results demonstrated potent antibacterial effects, particularly against Staphylococcus aureus, Micrococcus luteus, and Klebsiella pneumoniae. Furthermore, the cytotoxic potential of S. aethiopis was assessed on H-460 non-small cell lung cancer cells. The extract exhibited a dose-dependent cytotoxic effect, with a significant reduction in cell viability after 24 and 48 h of treatment, yielding IC50 values of 80.08 and 56.19 mu g/mL, respectively. These findings suggest that S. aethiopis possesses promising antimicrobial and anticancer properties, which could contribute to the development of novel therapeutic agents.
