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    ASSOCIATIONS BETWEEN PRL/RsaI POLYMORPHISM AND SOME PERFORMANCE TRAITS IN HOLSTEIN CATTLE REARED UNDER ORGANIC CONDITION
    (Pakistan Agricultural Scientists Forum, 2021) Ozdemir, M.; Sonmez, Z.; Aksakal, V.
    The aim of this study was to ascertain the Rsal polymorphism of the Prolactin (PRL) gene of 186 organically reared Holstein cattle by PCR-RFLP method, in order to reveal the genetic variation of the population, and to reveal the relationship between PRL/RsaI genotypes and some milk yield traits. In the study, the RsaI(-/-) genotype frequency, RsaI(+/+) genotype frequency and RsaI(-/+) genotype frequency of Holstein cattle were determined as 0.26, 0.22 and 0.52, respectively. The Rsal(-) gene frequency was 0.52, and the RsaI(+) gene frequency was 0.48. Hardy-Weinberg genetic equilibrium test showed that the distribution of genotype frequencies was determined to be in genetic equilibrium. The relationships between PRL/Rsal polymorphism and the examined milk yield traits were not significant. As a result, an economic and safe method PCR-RFLP for the detection of genetic markers, was successfully used in the determination of the 3 genotypes (RsaI(-/-), RsaI(-/+), and RsaI(+/+)) of Prolactin gene in Holstein cattle reared under organic condition, and the results was similar with previously reported literature.
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    The Relationship of Mitochondrial-derived Peptide (MOTS-c) and Brain Mitochondrial Carrier Protein 1 (BMCP1) Response in Sheep with Some Physiological Parameters
    (Agricultural Research Communication Centre, 2024) Bayraktar, B.; Demir, N.; Aksakal, V.
    Background: Mitochondria are organelles called power plants of the cell, which are found in eukaryotic cells and are responsible for the production of energy needed by the cells through aerobic respiration. Mitochondrial dysfunction causes endocrinological, cardiovascular, metabolic and neurodegenerative diseases. The aim of this study was to examine the effects of body condition score (BCS), lactation, pregnancy, age and gender on Mitochondrial-Derived Peptide (MOTS-c) and Brain Mitochondrial Transporter Protein 1 (BMCP1) levels in Akkaraman sheep. Methods: Sheep are classified into 3 categories according to BCS (BCS<2, BCS=3-3.5, BCS >= 4). In creating groups in terms of BCS, it was determined by the palpation method applied to the waist area. The sheep included in the study were divided into 4 groups: early lactation, early pregnancy, late pregnancy (dry period) and age (female and male sheep).1(st) group, ewes in the first period of pregnancy (on the 100(th) day of the first pregnancy) (n = 30), 2(nd) group, ewes in the early lactation period (n = 30), 3(rd) group, ewes in the dry period (the last two months of pregnancy) (n = 30) and in the 4(th) group, <2 (young) (n=30), 2-8 (adult) (n=30), 8> (old) (n=30), male and female sheep (n=30) of different ages. =180) total of 270 animals. Blood samples were taken from the jugular vein of the sheep before the morning feeding. MOTSc and BMCP1 levels were determined in blood serum samples by ELISA method. Result: In the study, serum MOTS-c and BMCP1 levels in sheep during lactation and pregnancy were determined to be lowest in the VKS<2 group and highest in the VKS >= 4 groups. Likewise, in age and gender groups, the highest level was detected in young lambs and yearlings in the VKS<2 group, while the highest level was detected in old sheep and rams in the VKS >= 4 group. As a result, it was determined that different age, gender, pregnancy and lactation periods affected serum MOTS-c and BMCP1 levels depending on the change in BCS (P<0.05). It was concluded that MOTS-c and BMCP1 may be useful parameters in the evaluation of mitochondrial function, energy metabolism and metabolic profile.