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    APPROPRIATE MACERATION DURATION FOR THE EXTRACTION OF PROPOLIS
    (Parlar Scientific Publications (P S P), 2019) Ecem-Bayram, Nesrin; Gercek, Yusuf Can
    The importance of the use of natural products as nutritional supplements in order to increase the quality of life and prevent diseases has increased in recent years. Among these products, propolis produced by honey bees is collected from plants and has a very complex structure in terms of chemical content. Propolis and its extracts have been used by humans for centuries because of its various bioactive properties. Although different methods can be used in the extraction stage, the most commonly used technique is extraction by maceration. However, there is no standard time period for extraction of propolis by maceration. We aimed to determine the changes in the concentrations of flavonoid group compounds pinocembrin, chrysin, tectochrysin, pinostrobin chalcone, gengwanin, naringenin and galangin from propolis samples exposed to seven different periods (1, 2, 5 10, 15, 20, 30 days) of maceration using GC-MS. The flavonoids pinocembrin, chrysin, tectochrysin, and naringenin reached their maximum concentrations at the end of 15 days of maceration and a decrease in their concentrations was observed after this period. The concentrations of other flavonoids did not show a steady increase or decrease with different maceration times. As a result, it can be said that the appropriate duration of maceration to extract the flavonoids responsible for propolis' numerous activities varies depending on the origin of the propolis and the nature of the compounds to be extracted.
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    Appropriate maceration duration for the extraction of propolis
    (Parlar Scientific Publications, 2019) Ecem-Bayram, Nesrin; Gercek, Ynsuf Can
    The importance of the use of natural products as nutritional supplements in order to increase the quality of life and prevent diseases has increased in recent years. Among these products, propolis produced by honey bees is collected from plants and has a very complex structure in terms of chemical content. Propolis and its extracts have been used by humans for centuries because of its various bioactive properties. Although different methods can be used in the extraction stage, the most commonly used technique is extraction by maceration. However, there is no standard time period for extraction of propolis by maceration. We aimed to determine the changes in the concentrations of flavonoid group compounds pinocembrin, chrysin, tectochrysin, pi-nostrobin chalcone, gengwanin, naringenin and galangin from propolis samples exposed to seven different periods (1, 2, 5 10, 15, 20, 30 days) of maceration using GC-MS. The flavonoids pinocembrin, chrysin, tectochrysin, and naringenin reached their maximum concentrations at the end of 15 days of maceration and a decrease in their concentrations was observed after this period. The concentrations of other flavonoids did not show a steady increase or decrease with different maceration times. As a result, it can be said that the appropriate duration of maceration to extract the flavonoids responsible for propolis' numerous activities varies depending on the origin of the propolis and the nature of the compounds to be extracted. © 2019 Parlar Scientific Publications. All rights reserved.
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    CHEMICAL CHARACTERIZATION OF KARS HONEY
    (Parlar Scientific Publications (P S P), 2018) Ozenirler, Cigdem; Ecem-Bayram, Nesrin; Gencay-Celemli, Omur; Celikbicak, Omur; Sorkun, Kadriye
    This study was undertaken to determine some chemical parameters (moisture, fructose/glucose ratio, hydroxymethylfurfural value, volatile compounds) of honey samples obtained from Kars. 100 honey samples from eight districts of Kars were evaluated. The chemical characterizations were as follows: average moisture (15,5 +/- 1,11%), HMF (0,77 ppm 0.9 ppm), F/G ratio (1,02 +/- 0,16). As a result of the gas chromatography mass spectrometry (GC MS) analyses, some volatile compounds in honey samples such as alcohols (1,15 +/- 1,84%), aldehydes (4,66 +/- 6,33%), aliphatic acids and their esters (1,86 +/- 4,5%), hydrocarbons (2,32 +/- 3,1%), flavonoids (4,34 +/- 1,63%), carboxylic acids and their esters (0,24 +/- 0,96%), ketones (6,05 +/- 6,35%), sugars (15,79 +/- 10,74%),vitamins (0,59 +/- 2,66%) were determined and quantitative variations of chemical components in different samples were reported.
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    Phenolic Acids in Pollen
    (Springer International Publishing, 2024) Kosti?, Aleksandar ?.; Gerçek, Yusuf Can; Ecem-Bayram, Nesrin
    Determination of phenolic composition of pollen has been one of the most popular topics over the past decade. Although most research studies focused on bee-collected pollen samples, it was also observed that phenolics, in particular, phenolic acids, are very important as protecting compounds in floral pollen grain, attached to sporopollenin. Based on the collected data a pronounced diversity of phenolic acids and derivatives was observed in pollen samples with different botanical/geographical origin. In total, 23 phenolic acids were identified/quantified in the samples with great predominance of the following ones: caffeic acid (0.04–450 ?g/g), ferulic acid (0.27–450 ?g/g) and p-coumaric acid (0.14–410 ?g/g). However, the highest content was observed for vanillic acid (4985.5 ?g/g) in the rape bee-collected pollen (China). In addition, several different derivatives, such as ethers, esters or amides, were also identified in pollen. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.
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    Pollen Chemistry & Biotechnology
    (Springer International Publishing, 2024) Ecem-Bayram, Nesrin; Kosti?, Aleksandar ?.; Gerçek, Yusuf Can
    Pollen is made up of many chemical constituents such as proteins, sugars, lipids, minerals and phytochemicals. These components make pollen products an excellent source of nutrients and antioxidants for consumers. However, mycotoxigenic fungi are also common in pollen, creating contamination from generated mycotoxins. With adequate monitoring, sampling, processing and storage of pollen the development of mycotoxins can be significantly prevented. Since pollen grain has strong membranes which can limit the bioavailability of some nutrients and phytochemicals, novel methods and pretreatments are required with special emphasis on microbiological pretreatment methodologies. Pollen Chemistry & Biotechnology summarizes current knowledge of the chemical composition of pollen, its importance as a functional food ingredient and its health promoting properties. As Introduction part a short elaborate about botanical characteristics and data, including morphology and anatomy of pollen and bee preferences, is given. Important factors such as the botanical and geographical origin, best practices for preservation of nutritional composition, bioactivity and safety of pollen are covered in full. The nutritional profile of pollen based on data for minerals, lipids and nutrients is presented. A detailed phytochemical profile of pollen based on data for phenolics, flavonoids, carotenoids, vitamins, bioactive compounds is also covered. Areas for the improvement of the bioavailability of different nutrients and bioactive compounds of pollen via applied pretreatments are presented, as are best practices for adequate pollen collection, procession and storage in order to obtain safe products. At the end, an overview about pollen bioactivity as reason to use it as source of pharmaceuticals is made. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.
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    Pollen Morphology and Anatomy with Botanical Preferences Made by Bees: An Introduction Data
    (Springer International Publishing, 2024) Canlı, Deniz; Ecem-Bayram, Nesrin
    Pollen is a microspore containing vegetative and generative cells prepared for pollination in seed plants. Because pollen grains show highly variable characteristics, these structures are used to evaluate kinship relationships and similarity levels in plants. However, plant sources of honey, which is consumed as an important food source and functional food, can also be determined by identifying the pollen grains included in the honey by bees. In this study, the characteristic parameters used for the identification of pollen grains in plants and the plant sources frequently visited by bees were mentioned. As a result, variables such as size, polarity and symmetry, shape, exine sculpturing, intine properties and apertures are the main characteristics used to describe pollen grains. Considering these variables, it can contribute to the identification of plant taxa. In addition, the plants that honey bees visit to collect nectar and the botanical origin/source of honey (monofloral-multifloral) can also be determined by identifying the pollen grains included in the honey. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.
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    The Effect of Different Drying Methods on Bioactive and Nutrition Contents of Bee Bread and Mathematical Modeling of Drying Characteristics
    (John Wiley and Sons Inc, 2023) Kutlu, Naciye; Gerçek, Yusuf Can; Ecem-Bayram, Nesrin
    In this study, the aim was to determine the effect of different drying methods (with microwave and hot air) on the color, nutrient and bioactive contents of fresh bee bread. Drying characteristics were also investigated. Microwave and hot air drying were applied at different microwave powers and temperatures, respectively. Lower moisture ratios and highest effective diffusion coefficients were obtained with microwave drying in a shorter time. The Midilli model was found to be the most suitable thin-layer drying model for both methods. Regardless of the drying conditions, moisture, ash, protein, carbohydrate, and lipid proportions were observed to vary in the ranges of 4.9–8.2 %, 1.61–2.67 %, 17.47–32.54 %, 39.92–60.84 %, and 7.10–8.89 %, respectively. The lowest color difference was obtained for the sample dried at 210 W. As a result, it was determined that microwave drying is more suitable for preserving the nutritional and bioactive content of bee bread during drying. © 2022 Wiley-VHCA AG, Zurich, Switzerland.