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    Characterization of the black cumin (Nigella sativa L.) honey from Turkiye
    (Elsevier, 2023) Kemal, Mehmet; Esertas, Ulku Zeynep Ureyen; Kanbur, Esra Demir; Kara, Yakup; Ozcelik, Ali Erdem; Can, Zehra; Kolayli, Sevgi
    This case report study investigated the botanical, physicochemical, and phenolic compositions, as well as the antioxidant and antimicrobial properties of varying amounts of black cumin honey (Nigella sativa) from Burdur region of Turkiye. The samples had an average moisture value of 17.23% and a Nigella sativa pollen ratio ranging from 19% to 51%. Electrical conductivity (EC) values ranged from 0.36 to 0.54 mS/cm. The average color values of Hunter (L, a, b) were 43.55 (L*), 32.77 (a*), and 64.52 (b*), respectively. The proline contents were ranged from 875 to 1624 mg/kg. The average F/G ratio and F+G value were 1.43 +/- 0.11 and 68.75 +/- 4.75, respectively. The average of total phenolic (TP) and total flavonoid (TF) contents were 36.87 +/- 7.00 mg GAE/100 g and 5.63 +/- 1.45 mg QUE/100 g, respectively. The antioxidant capacities of the samples were tested using the ferric reducing/antioxidant power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays, with mean values of 179.40 +/- 25.35 mu mol FeSO4 center dot 7H(2)O/100 g and 46.07 +/- 12.87 mg/mL, respectively. The phenolic composition of the samples were tested using the HPLC-PDA method with 25 phenolic standards. Gallic acid, p-OH benzoic acid, t-cinnamic acid, p-coumaric acid, chrysin, and pinocembrin were determined as common components. The samples exhibited high antimicrobial activities against Staphylococcus aureus, Escherichia coli, Chromobacterium violaceum, Bacillus cereus, Klebsiella pneumoniae, and Acinetobacter haemolyticus, but did not affect Candida albicans, Candida parapilopsis, or Mycobacterium smegmatis.
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    Determination of phenolic content and bioactive characterization of Anatolian propolis
    (Springer, 2024) Can, Zehra; Birinci, Ceren; Kara, Yakup; Esertas, Ulku Zeynep Ureyen; Kolayli, Sevgi
    In this study, propolis referred to as of Turkiye Anatolian propolis was utilized. This work was aim to prepare a 70% ethanolic extract of propolis and subsequently determine certain biochemical parameters, total phenolic content (TPC), total flavonoid content (TFC), phenolic and flavonoid composition, inhibitory effects on certain enzymes [acetylcholinesterase (AChE), urease, and alpha-amylase], along with assessments of antiviral activity against Vero cells, lung cancer cells (A549), and breast cancer cells (MDA-MB-231). The richness of phenolic content in Anatolian propolis clearly indicates a high inhibitory effect on these enzymes. Additionally, it was observed to exert significant effects on cancer cells and demonstrated effective antiviral activity. It can be contemplated that the Anatolian propolis might serve as a potential source for novel drugs, and the isolation of its compounds could offer opportunities for utilization in traditional medicine.