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Genotypic Identification of Lactic Acid Bacteria in Pastirma Produced w h Different Curing Processes
(Kafkas Univ, Veteriner Fakultesi Dergisi, 2019) Cinar, Kubra; Fettahoglu, Kubra; Kaban, Guzin
The lactic acid bacteria isolated from pastirma, produced under controlled conditions using two different curing temperatures (4 degrees C or 10 degrees C) and two different curing agents (150 mg/kg sodium nitrite or 300 mg/kg potassium nitrate), were subjected to genotypic (16S rRNA sequecing) identification. According to the identification results, 68 of 87 isolates (78.16%) was identified as Pediococcus pentosaceus. This species was followed by P. acidilactici (14.94%), Lactobacillus sakei (4.60%) and L plantarum (2.30%), respectively. P pentosaceus was dominant species in all curing applications (4 degrees C/nitrate or nitrite or 10 degrees C/nitrate or nitrite). Another species determined in all groups was P. acidilactici. While L. plantarum was only isolated from samples produced with nitrate (4 degrees C or 10 degrees C), L sakei was isolated from samples produced with nitrite (4 degrees C or 10 degrees C). The effect of the curing agent on the biodiversity of lactic acid bacteria in pastirma was more effective than the curing temperature.