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Öğe An Integrated In Vitro and In Silico Investigation of the Bioactive Properties of Wild Glycyrrhiza glabra var. glandulifera(Springer, 2025) Kizil, Hamit Emre; Ulcay, Sibel; Ekincioglu, Yavuz; Ogutcu, Hatice; Agar, GulerayThis investigation examined methanol extracts obtained from the whole plant (encompassing all organs except roots), flowers, and fruits of wild Glycyrrhiza glabra var. glandulifera, a liquorice cultivar indigenous to the Cappadocia region of T & uuml;rkiye, constituting the first comprehensive in vitro and in silico analysis of this species within the region. Anatomical identification revealed distinctive structural characteristics, including thick-walled epidermal cells in the stem, organized clusters of collenchyma cells, and well-defined sclerenchyma layers encompassing the vascular bundles. The petiole exhibited a characteristic heart-shaped morphology containing three vascular bundles, while foliar analysis demonstrated the presence of stomata on both adaxial and abaxial surfaces, accompanied by a bifacial mesophyll arrangement. The extracts demonstrated remarkable antimicrobial efficacy against both Gram-positive and Gram-negative bacteria, as well as various fungal species. Furthermore, significant cytotoxic activity was observed against non-small cell lung cancer (H460), with the whole plant extract exhibiting IC50 values of 117.8 mu g/mL (24 h) and 105.6 mu g/mL (48 h). Flower extracts demonstrated IC50 values of 116.8 mu g/mL (24 h) and 112.7 mu g/mL (48 h), while fruit extracts displayed superior potency with IC50 values of 104.4 mu g/mL (24 h) and 63.09 mu g/mL (48 h). Molecular docking analyses, conducted utilizing AutoDock Tools 1.5.6 and BIOVIA Discovery Studio Visualizer 4.5, revealed significant protein-ligand interactions, yielding optimal binding energy and inhibition constant values of -9.57 kcal/mol and 96.92 nM, respectively. The RMSD values were calculated based on structural variations between ligand-protein complexes during re-docking procedures. These findings underscore the substantial biological potential of Glycyrrhiza glabra var. glandulifera from Cappadocia, necessitating further comprehensive investigation.Öğe Anti-genotoxic Effects of Schiff bases and their Mn(III) Complexes Containing L-Aspartic acid and L-Phenylalanine(Gazi Univ, 2018) Hasanoglu Ozkan, Elvan; Kizil, Hamit Emre; Sakiyan, Iffet; Nartop, Dilek; Sari, Nursen; Agar, GulerayThe purpose of the research was to evaluate the genotoxic and anti-genotoxic properties of Schiff bases and their Mn (III) complexes containing L-aspartic acid, and L-phenylalanine. The anti-genotoxic properties of four compounds in human lymphocytes cells were investigated by sister chromatid exchanges (SCEs) test system against aflatoxin Bi (AFBi). The results showed that compounds have strong anti-genotoxic properties.Öğe Antiproliferative and apoptotic properties of vulpinic acid on A549, AGS and HeLa cells(Elsevier Science Bv, 2017) Kizil, Hamit Emre; Agar, Guleray[Abstract Not Available]Öğe Antiproliferative effects of Evernic acid on A549 and healthy human cells: An in vitro study(Elsevier Science Bv, 2015) Kizil, Hamit Emre; Agar, Guleray; Anar, Mustafa[Abstract Not Available]Öğe Biosynthesis of Functionalized Silver Nanomaterials Utilizing Seeds of Black Rosehip (Rosa pimpinellifolia L.) and its Remarkable Antioxidative Capacity(Agricultural Research Communication Centre, 2025) Kizil, Hamit EmreBackground: Silver nanoparticles (AgNPs) have gained significant attention due to their unique antimicrobial, catalytic and optical properties. This study focuses on the green synthesis of silver nanoparticles using black rosehip (Rosa pimpinellifolia L.) seeds (Rp-AgNPs) and evaluates their biological activities. Methods: Rp-AgNPs were synthesized using R. pimpinellifolia seed extract and characterized using multiple analytical techniques including UV-Vis spectroscopy, XRD, FTIR, TEM, SEM, EDAX, zeta potential analysis, DSC, SERS and XPS. The biological activities were assessed through DPPH free radical scavenging assay, cytotoxicity testing against H460 lung cancer cells and antimicrobial activity evaluation against various pathogens. Result: The synthesized Rp-AgNPs exhibited spherical morphology with an average size of 51.27 nm and demonstrated stability with a zeta potential of-18.9. The nanoparticles showed enhanced antioxidant activity (IC50 = 30.89 mu g/mL) compared to the seed extract (IC50 = 136.4 mu g/mL). Cytotoxicity studies revealed significant effects against H460 cells with an IC50 of 65.8 mu g/mL. Antimicrobial testing demonstrated effectiveness against both Gram-positive and Gram-negative bacteria (MIC 50-100 mu g/mL) and Candida strains (MIC 100-200 mu g/mL).Öğe Can Enzymatic Modified Pectin in Mediterranean Fruits Be Therapeutic Against Stomach Cancer?(Wiley, 2024) Ustun, Selime; Kizil, Hamit Emre; Dertli, EnesThe aim of this study was to evaluate the cytotoxic effects of enzymatically modified pectin products derived from grapefruit, jujube, and kumquat on the MKN-45 gastric cancer cell line in vitro. FTIR analysis revealed that the spectra of the pectins produced were comparable to those of commercial pectin and included the characteristic peaks identified in the literature. The galacturonic acid content was measured as 612 mg/g in grapefruit pectin, 544 mg/g in jujube pectin, and 704 mg/g in kumquat pectin. DSC analysis indicated that all pectin samples and their modifications exhibited one endothermic peak and one exothermic peak. Cytotoxicity assessments revealed that the enzymatically modified grapefruit pectin demonstrated significant cytotoxic effects across all tested concentrations, with 0.075 mg/mL being the most effective. For kumquat pectin, all tested concentrations showed cytotoxic properties, with 0.3, 0.15, and 0.075 mg/mL being the most effective. In the case of jujube pectin, cytotoxic effects were observed at all concentrations except for 1.2 mg/mL. In vitro cytotoxic effects of enzymatically modified pectin products from grapefruit, jujube and kumquat on MKN-45 gastric cancer cell line at different concentrations were determined.imageÖğe Cytotoxic and antiproliferative effects of evernic acid on HeLa cell lines: A candidate anticancer drug(Elsevier Science Bv, 2014) Kizil, Hamit Emre; Agar, Guleray; Anar, Mustafa[Abstract Not Available]Öğe DETERMINATION OF ANTIGENOTOXIC EFFECTS OF FOUR LICHEN SPECIES BY USING HUMAN LYMPHOCYTES(Parlar Scientific Publications (P S P), 2015) Anar, Mustafa; Aslan, Ali; Ceker, Selcuk; Kizil, Hamit Emre; Alpsoy, Lokman; Agar, GulerayIn this study, the geno-toxic and antigeno-toxic properties of the total extracts of four lichen species were investigated by using human lymphocytes. We obtained total extracts from R. farinacea, X parietina, U articulata and U. filipendula lichen species. The results of our studies showed that 5 mu M concentrations of AFB(1) changed the frequencies of micronucleus (MN) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GPx) activities. When 5, 10 and 20 mu g/ml concentrations of total extract were added to AFB(1), the frequencies of MN and MDA levels were decreased, and SOD, GSH and GPx levels were increased. Consequently, our findings show that four lichen total extracts have strong antigenotoxic properties against aflatoxin B-1.Öğe The determination of antioxidative effect and anticancer potential of vulpinic acid(Elsevier Science Bv, 2014) Anar, Mustafa; Kizil, Hamit Emre; Agar, Guleray[Abstract Not Available]Öğe Determination of cytotoxic and proliferative effects of 2-amino-5-nitrobenzonitrile on AGS cancer cell line(Elsevier Science Bv, 2015) Anar, Mustafa; Kizil, Hamit Emre; Agar, Guleray[Abstract Not Available]Öğe Determination of the Antigenotoxic effects of Evernic acid against genotoxic effects of CCL4 on HUVEC lines(Elsevier Science Bv, 2017) Agar, Guleray; Kizil, Hamit Emre; Ceker, Selcuk; Capik, Ozel; Sengul, Bulent[Abstract Not Available]Öğe Dietary Carvacrol Attenuates Cyclophosphamide-Induced Neurotoxicity: Implications for Food-Derived Neuroprotection and Molecular Mechanisms(Wiley, 2025) Kizil, Hamit EmreCarvacrol (CRV) is a phenolic monoterpene abundant in culinary herbs such as oregano and thyme and is well known for its potent antioxidant, anti-inflammatory, and neuroprotective properties. This study investigated the ability of CRV to counteract neurotoxicity induced by cyclophosphamide (CP), a widely used antineoplastic agent. Male Wistar albino rats were divided into five groups and received CP and/or CRV treatments. Neurotoxicity and neuroprotection were evaluated through biochemical assays, real-time PCR, histopathological and immunohistochemical analyses, and behavioral testing (Morris Water Maze). CP administration led to significant increases in oxidative stress markers, disruption of antioxidant enzyme activities, upregulation of inflammatory mediators (NF-kappa B, TNF-alpha, iNOS), dysregulation of apoptotic regulators (increased Bax and Casp-3, decreased Bcl-2), alterations in autophagy markers (Beclin-1, LC3A, LC3B), and suppression of Notch1/Hes1 signaling. Histopathological analyses revealed neuronal degeneration, vascular hyperemia, and increased GFAP and 8-OHdG expression in brain tissue. CRV treatment, particularly at higher doses, effectively mitigated these biochemical, molecular, and histological alterations. Notably, CRV administration preserved spatial learning and memory function in CP-treated rats, as demonstrated by the Morris Water Maze test, indicating functional neuroprotection. These findings highlight the multifaceted neuroprotective mechanisms of CRV and suggest its potential as a food-derived bioactive compound for development into functional foods or dietary supplements to improve the quality of life for cancer patients undergoing chemotherapy.Öğe Morin ameliorates methotrexate-induced hepatotoxicity via targeting Nrf2/HO-1 and Bax/Bcl2/Caspase-3 signaling pathways(Springer, 2023) Kizil, Hamit Emre; Caglayan, Cuneyt; Darendelioglu, Ekrem; Ayna, Adnan; Gur, Cihan; Kandemir, Fatih Mehmet; Kucukler, SefaBackgroundOrgan toxicity limits the therapeutic efficacy of methotrexate (MTX), an anti-metabolite therapeutic that is frequently used as an anti-cancer and immunosuppressive medicine. Hepatocellular toxicity is among the most severe side effects of long-term MTX use. The present study unveils new confirmations as regards the remedial effects of morin on MTX-induced hepatocellular injury through regulation of oxidative stress, apoptosis and MAPK signaling.Methods and resultsRats were subjected to oral treatment of morin (50 and 100 mg/kg body weight) for 10 days. Hepatotoxicity was induced by single intraperitoneal injection of MTX (20 mg/kg body weight) on the 5th day. MTX related hepatic injury was associated with increased MDA while decreased GSH levels, the activities of endogen antioxidants (glutathione peroxidase, superoxide dismutase and catalase) and mRNA levels of HO-1 and Nrf2 in the hepatic tissue. MTX treatment also resulted in apoptosis in the liver tissue via increasing mRNA transcript levels of Bax, caspase-3, Apaf-1 and downregulation of Bcl-2. Conversely, treatment with morin at different doses (50 and 100 mg/kg) considerably mitigated MTX-induced oxidative stress and apoptosis in the liver tissue. Morin also mitigated MTX-induced increases of ALT, ALP and AST levels, downregulated mRNA expressions of matrix metalloproteinases (MMP-2 and MMP-9), MAPK14 and MAPK15, JNK, Akt2 and FOXO1 genes.ConclusionAccording to the findings of this study, morin may be a potential way to shield the liver tissue from the oxidative damage and apoptosis.Öğe Pharmacological Potential and Bioactive Components of Wild Anatolian Sage (Salvia aethiopis L.)(Wiley, 2025) Bilginoglu, Emine; Kizil, Hamit Emre; Ogutcu, Hatice; Agar, Guleray; Bagci, YavuzSalvia aethiopis L., a member of the Lamiaceae family, has gained attention due to its bioactive compounds with potential therapeutic applications. This study investigated the chemical composition and biological activities of S. aethiopis ethanol extract collected from Karaman province, T & uuml;rkiye. The phenolic profile of the plant was characterized using LC-MS/MS analysis, which revealed substantial amounts of rosmarinic acid, ferulic acid, caffeic acid, and p-coumaric acid. The antimicrobial activity of the extracts was evaluated against various Gram-positive and Gram-negative bacteria, as well as fungal strains, using the agar well diffusion method. The results demonstrated potent antibacterial effects, particularly against Staphylococcus aureus, Micrococcus luteus, and Klebsiella pneumoniae. Furthermore, the cytotoxic potential of S. aethiopis was assessed on H-460 non-small cell lung cancer cells. The extract exhibited a dose-dependent cytotoxic effect, with a significant reduction in cell viability after 24 and 48 h of treatment, yielding IC50 values of 80.08 and 56.19 mu g/mL, respectively. These findings suggest that S. aethiopis possesses promising antimicrobial and anticancer properties, which could contribute to the development of novel therapeutic agents.
