Yazar "Topcu, Kubra Cinar" seçeneğine göre listele
Listeleniyor 1 - 6 / 6
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Assessment of technological attributes of autochthonous starter cultures in Turkish dry fermented sausage (sucuk)(Wiley, 2022) Kaban, Guzin; Sallan, Selen; Topcu, Kubra Cinar; Borekci, Bilge Sayin; Kaya, MukerremThe study aims to evaluate the technological properties of autochthonous strains (Lactobacillus sakei S15, Lactobacillus plantarum S24, L. plantarum S91, Pediococcus pentosaceus S128b and Staphylococcus carnosus G109) in Turkish dry fermented sausage (sucuk). After 24 h of fermentaPtion, all lactic acid bacteria strains reduced the pH to below 5.0, while the pH in the control group was above 5.3. The number of lactic acid bacteria strains reached 10(8)-10(9) cfu g(-1) during fermentation. Staphylococcus carnosus G109 remained at the inoculation level of 10(6) cfu g(-1) during ripening. Lactobacillus sakei S15 as mono-culture showed higher TBARS values compared to other strains. The control group had the lowest L* value and autochthonous strains caused no significant difference for a* value. According to principal component analysis results, most volatile compounds were positively correlated with the group containing only L. sakei S15.Öğe Effect of tarragon addition on volatile compound profile and some quality paramaters of sucuk(Pamukkale Univ, 2021) Kamiloglu, Aybike; Elbir, Tugba; Topcu, Kubra CinarThe goal of the present study was to investigate the effect of tarragon and ripening period on certain quality parameters and the volatile profile of sucuk (Turkish dry fermented sausage). Tarragon was used in sucuk samples at three distinct levels (0.25%, 0.50% and 0.75%), and tarragon-free samples were used as control. Analyses were performed on the 0., 3., 5., 7., 9. and 13. days of ripening time. Although the presence of tarragon did not affect L* and b* values, the pH, a* (P<0.01), aw (P<0.05), and TBARS values of the sucuk samples were statistically affected. Tarragon demonstrated antioxidant activity in sucuk in accordance with 90 % +/- 0.3 DPPH scavenging activity. When the volatile profile was evaluated, the key component affected by the addition of tarragon was determined as Anisole, p-allyl-. As a result, while an antioxidative effect was detected during ripening in the presence of tarragon in sausage, undesirable changes were not observed on some quality characteristics such as pH and a(w).Öğe Genotypic and Technological Characterization of Lactic Acid Bacteria and Coagulase-Negative Staphylococci Isolated from Sucuk: A Preliminary Screening of Potential Starter Cultures(Mdpi, 2025) Kaya, Mukerrem; Sayin, Bilge; Topcu, Kubra Cinar; Karadayi, Mehmet; Kamiloglu, Aybike; Gulluce, Medine; Kaban, GuzinThis study aimed to characterize lactic acid bacteria (LAB) and coagulase-negative staphylococci (CoNS) isolated from traditionally produced sucuk for their potential use in starter culture development and food safety applications in fermented meat products. A total of 145 isolates (95 LAB and 50 CoNS) were analyzed through genetic identification, phylogenetic analysis, and assessments of technological properties. Antagonistic activity against Listeria monocytogenes and Staphylococcus aureus was also evaluated, along with antibiotic sensitivity. Among LAB, Lactiplantibacillus plantarum was the most prevalent species (60 isolates), while Staphylococcus xylosus was the predominant CoNS species (24 isolates). The isolates exhibited diverse technological properties and varying levels of antagonistic activity against the tested pathogens. Antibiotic sensitivity tests indicated that 15 selected isolates were negative for antibiotic resistance genes. Overall, this comprehensive characterization provides valuable insights for the development of starter cultures and for enhancing food safety in fermented meat products.Öğe Optimization of Ethanol Extraction Conditions From Sun-Dried Apricot and Prediction of Antioxidant, Phenolic, and Flavonoid Contents and Their Effects on HCT116 Cells Using Artificial Neural Networks(Wiley, 2025) Topcu, Kubra Cinar; Gueven, Sara Altun; Cakir, Ozlem; Anlar, Pinar; Durul, Melekber Sulusoglu; Ay, Mizgin; Ercisli, SezaiThis study aimed to optimize the ethanol extraction conditions of sun-dried apricot (Prunus armeniaca L.) and evaluate the antioxidant capacity, phenolic, and flavonoid contents of the obtained extracts. Response surface methodology (RSM) was applied to determine the optimal extraction conditions, which were identified as 60 degrees C temperature, 34% ultrasonic power, 46 min sonication time, and a 4 g/mL solid-liquid ratio. Under these conditions, the extract exhibited a total phenolic content (TPC) of 4.20 mg GAE/g, a total flavonoid content (TFC) of 7.09 mg QE/g, a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging capacity of 1.37 mg TE/g, a ferric reducing antioxidant power (FRAP) value of 9.12 mg TE/g, and a thiobarbituric acid reactive substances (TBARS) level of 1.69 mg MDA/g. The artificial neural network (ANN) model provided highly accurate predictions for these parameters. Additionally, cell culture experiments demonstrated that the extract exerted a dose-dependent cytotoxic effect on HCT116 colon cancer cells, significantly reducing their viability. These findings highlight the potential of sun-dried apricot extracts as natural antioxidants with possible applications in the functional food and pharmaceutical industries.Öğe Probiotic properties of lactic acid bacteria strains isolated from pastirma(Elsevier, 2020) Topcu, Kubra Cinar; Kaya, Mukerrem; Kaban, GuzinIn this study, the probiotic properties of 80 lactic acid bacteria (LAB) strains isolated from pastirma, a dry-cured meat product, were investigated. For this purpose, strains were investigated for their resistance to bile salt and hydrophobicity abilities. In 6 strains selected as a result of these analyses, development at high salt concentrations, auto-aggregation and co-aggregation, resistance to low pH, and adhesion analyses were performed. The growth percentage of all strains decreased with increasing bile salt concentration at both 25 degrees C and 37 degrees C. P. pentosaceus K7, K41, K44, K51 and K81 and P. acidilactici K99 gave the highest values in terms of hydrophobicity ability. The highest auto-aggregation and the highest co-aggregation were found in P. pentosaceus K41 (17.62%) and P. pentosaceus K44 strain (26.23%), respectively. These six strains showed tolerance to high acidity even at pH 2. The strains significantly maintained their viability in the simulated gastric and intestinal fluids. Furthermore, the strains had low adhesion capacity in Caco-2 and HT-29 cell lines and generally showed resistance under gastrointestinal system conditions. As a result, P. pentosaceus K44, P. acidilactici K99 and P. pentosaceus K41 could be considered as probiotic strains.Öğe Sensitive and selective nitrite sensor based on CuCo2O4-rGO nanocomposite synthesized by one-pot electrochemical method in pastırma(Elsevier Science Sa, 2025) Daldal, Pinar Anlar; Dogan, Hulya Ozturk; Topcu, Kubra CinarIn this study, CuCo2O4-rGO nanostructures were synthesized by a one-pot electrochemical method on the surface of Pencil Graphite Electrode (PGE) for nitrite determination in past & imath;rma, a traditional meat product. The developed modified electrode was used to determine the amount of nitrite in past & imath;rma samples obtained from three different brands in the Turkish market. When the nitrite values obtained by the electrochemical method were compared with the spectrophotometric method, they showed 91.20 % to 100 % agreement. In addition, the physicochemical properties of past & imath;rma samples, such as pH, moisture, protein, fat, and ash, were analysed, and it was determined that pH and moisture values are critical parameters for product quality. These results show that the developed electrode provides a practical and sensitive method for nitrite sensing.
