A novel bacteriocin produced by Lactobacillus delbrueckii subsp. lactis with a specifically antimicrobial activity against Bacillus cereus

Basit Öğe Kaydı
dc.authorid0000-0002-1837-5682en_US
dc.contributor.authorKAYA, HALİL İBRAHİM
dc.contributor.authorŞİMŞEK, ÖMER
dc.date.accessioned2020-11-25T11:25:41Z
dc.date.available2020-11-25T11:25:41Z
dc.date.issued2017en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractBacteriocins are the ribosomal synthesized antimicrobial peptides used as natural preservatives against pathogens during food production process. The main problem of using bacteriocin and their producers is that these antimicrobials also inhibit the existing flora including non-starter lactic acid bacteria. The question is whether bacteriocins with narrow antimicrobial activity could be a solution? In this study, bacteriocins produced from lactic acid bacteria (LAB) having antimicrobial activity specifically on Bacillus cereus (BC) was isolated and characterized. To select the anti-BC bacteriocin LAB producer, a cocktail including of diverse B. cereus strains was used as indicators. The isolate Yo6B1 obtained from condensed yogurt showed antimicrobial activity against BC at both spot and well diffusion assays. This isolate and its bacteriocin-like metabolite did not inhibit any LAB tested, although it had antimicrobial activity against all BC strains (n=59) isolated from food samples. 16S rDNA sequence of Yo6B1 indicated that it was 99% homolog with the Lactobacillus delbrueckii subsp. lactis. The partial purified Yo6B1 was degraded with proteolytic enzymes including trypsine, α-chmotrypsine, pepsine and proteinase K proved that Yo6B1 was a bacteriocin although the genome of Lb. delbrueckii subsp. lactis did not contain any known bacteriocin production genes. MALDI-ToF analysis indicated that bacteriocin Yo6B1 has a small molecular size. As a conclusion, Lb. delbrueckii subsp. lactis produced a novel narrow spectrum bacteriocin, which has specifically antimicrobial activity against BC. This study also demonstrated that pathogen-specific bacteriocins could be used to eliminate pathogens in foods with no antibacterial effects against LAB microflora.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12403/2182
dc.institutionauthorKAYA, HALİL İBRAHİM
dc.language.isoenen_US
dc.relation.ispartof12th International Symposium on Lactic Acid Bacteriaen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLactic Acid Bacteriaen_US
dc.subjectBacteriocinen_US
dc.subjectPathogenen_US
dc.subjectAntimicrobialen_US
dc.titleA novel bacteriocin produced by Lactobacillus delbrueckii subsp. lactis with a specifically antimicrobial activity against Bacillus cereusen_US
dc.typeConference Objecten_US

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Gıda Mühendisliği Bölümü Koleksiyonu