Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates, and Lignins

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Tarih

2015

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Elsevier

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavior in solution. This chapter considers the relevant analytical ultracentrifuge methodology available for characterizing these and related carbohydrate-based systems and information this methodology supplies, in terms of sizes, shapes, and interactions using a comprehensive range of examples, including glycoconjugates and lignins. The relevance and potential of recent software developments such as SEDFIT-MSTAR, the Extended Fujita algorithm, and HYDFIT are considered.

Açıklama

Anahtar Kelimeler

Size,Shape, Interactions, SEDFIT-MSTAR, Extended Fujita, Flexibility

Kaynak

Methods in Enzymology

WoS Q Değeri

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Cilt

Sayı

562

Künye