Recent Advances in the Analysis of Macromolecular Interactions Using the Matrix-Free Method of Sedimentation in the Analytical Ultracentrifuge

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dc.authorid0000-0002-2150-2726en_US
dc.contributor.authorHarding, S.E., Gillis, R.B., Almutairi, F., Erten, T., Kök, M.Ş., Adams, G.G.,
dc.date.accessioned2020-12-04T09:45:47Z
dc.date.available2020-12-04T09:45:47Z
dc.date.issued2015en_US
dc.departmentFakülteler, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümüen_US
dc.description.abstractSedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactionsen_US
dc.identifier.doi10.3390/biology4010237
dc.identifier.endpage250en_US
dc.identifier.issue4en_US
dc.identifier.startpage237en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12403/2284
dc.institutionauthorErten, Tayyibe
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofBiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergien_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcarbohydrate; nucleic acid; interaction; hydrodynamicsen_US
dc.titleRecent Advances in the Analysis of Macromolecular Interactions Using the Matrix-Free Method of Sedimentation in the Analytical Ultracentrifugeen_US
dc.typeArticleen_US

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