DSpace Arşivi :: by Yazar "Can, Zehra" değerine göre listeleniyor

Yazar "Can, Zehra" seçeneğine göre listele

Listeleniyor 1 - 20 / 47

A Promising Antibacterial Inhibition of Propolis Extracts Against Carbapenem-Resistant Bacteria Isolated From Clinical Samples
(Wiley-V C H Verlag Gmbh, 2025) Esertas, Ulku Zeynep; Inal, Nese; Can, Zehra; Kolayli, Sevgi
The increasing inadequacy of antibiotics against multidrug-resistant pathogens is a growing concern. In this study, the antibacterial activity of propolis extracts with different phenolic profiles against carbapenem-resistant clinical isolates was investigated. The antimicrobial activity of propolis extract was tested against Acinetobacter baumannii (K17, K16, and K21), Klebsiella pneumoniae (K22, and K19), and Stenotrophomonas maltophilia (E5, E7, and E4) bacterial strains isolated from tracheal aspirate and blood cultures. The total phenolic content and phenolic composition of the extracts were analyzed. Antimicrobial activity values examined according to the disk diffusion method were measured as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The results showed that all propolis extracts showed different inhibition values against bacterial strains. As a result, the high inhibitory potential of the propolis extracts against resistant bacteria such as A. baumannii, K. pneumoniae, and S. maltophilia is promising for combating infections. However, the study needs to be supported by further randomized clinical studies.
Antioxidant Activities of different Peanut (Arachis hypogaea L.) Market Types by Spectrophotometric Techniques Combined with Chemometrics
(Wiley-V C H Verlag Gmbh, 2024) Gidik, Betuel; Can, Zehra; Oenemli, Fadul
Peanut is rich in oil and protein and has a large content of bioactive constituents consisting of tocopherols, phytosterols, and so on. Generally, Virginia, Spanish, Valencia and Runner market types are grown of peanut. In this study, it is aimed to determine the antioxidant activity, total phenolic content and total flavonoid content of peanuts from four different market types, for the first time, and group them with principal component analysis (PCA) and hierarchical cluster analysis (HCA). For PCA, PC1 and PC2 explained 87.655 % of the total variation and, according to the HCA of peanut samples, two main groups were determined. The total phenolic content changed 1.556 to 2.899 mg GAE/g. The lowest value have seen at Spanish merket type to determine the antioxidant activities of peanut samples were maked FRAP and DPPH assay, the lowest FRAP value (8.136 mu mol FeSO(4)7H(2)O/g sample) was seen at Valencia market type, the highest (14.004 mu mol FeSO(4)7H(2)O/g sample) was seen at Virginia market type. It was determined that the total flavonoid, total phenolic content, and antioxidant activities of the Virginia, Valencia, Spanish, and Runner market types included in the study were different from each other, and the Virginia market type showed superior characteristics compared to the others. The results obtained suggest that Virginia market type may be preferred more especially in peanut cultivation for food uses. It is thought that this study can be a source for future studies by eliminating a deficiency in the literature.
Antioxidant activity and phenolic composition of propolis from Marmara region, Turkey
(Taylor & Francis Ltd, 2024) Can, Zehra; Kara, Yakup; Kolayli, Sevgi; cakmak, Ibrahim
This study investigated various properties of raw propolis samples from Marmara region of Turkey. Total phenolic content (TPF), total flavonoid (TFC) values, condensed tannin content (CTC) and phenolic profiles of ethanolic extracts (70%) were analyzed. Antioxidant activities were tested using the Ferric (III) reduction/antioxidant capacity (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging methods. Phenolic profiles of the samples were analyzed using high performance liquid chromatography (RP-HPLC-UV) method. TPC, TFC and CTC values in the samples ranged from 52.9 to 203.5 mg GAE/g, 13.6 to 43.9 mg QUE/g, and 2.0 and 2.9 mg CTE/g, respectively. Caffeic acid phenyl ester (CAPE), chrysin, pinocembrin, rutin, and t-cinnamic acid were detected as the common phenolic components in all samples. In conclusion, Marmara region propolis is a rich source of bioactive compounds, especially flavonoids with antioxidant properties.
Antioxidant activity and phenolic composition of propolis from Marmara region, Turkey
(Taylor and Francis Ltd., 2024) Can, Zehra; Kara, Yakup; Kolaylı, Sevgi; Çakmak, Ibrahim
This study investigated various properties of raw propolis samples from Marmara region of Turkey. Total phenolic content (TPF), total flavonoid (TFC) values, condensed tannin content (CTC) and phenolic profiles of ethanolic extracts (70%) were analyzed. Antioxidant activities were tested using the Ferric (III) reduction/antioxidant capacity (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging methods. Phenolic profiles of the samples were analyzed using high performance liquid chromatography (RP-HPLC-UV) method. TPC, TFC and CTC values in the samples ranged from 52.9 to 203.5 mg GAE/g, 13.6 to 43.9 mg QUE/g, and 2.0 and 2.9 mg CTE/g, respectively. Caffeic acid phenyl ester (CAPE), chrysin, pinocembrin, rutin, and t-cinnamic acid were detected as the common phenolic components in all samples. In conclusion, Marmara region propolis is a rich source of bioactive compounds, especially flavonoids with antioxidant properties. © 2023 International Bee Research Association.
Antioxidant activity and phenolic content of bee breads from different regions of Türkiye by chemometric analysis (PCA and HCA)
(Springer, 2024) Can, Zehra; Gidik, Betul; Kara, Yakup; Kolayli, Sevgi
The objective of this study was to assess the antioxidant activity and phenolic compounds of bee bread samples obtained from various regions in T & uuml;rkiye The goal was to characterize and classify 15 of bee bread samples based on their geographical origins. This investigation employed chemometric techniques, specifically principal component analysis (PCA) and a hierarchical clustering algorithm (HCA), for the inaugural comprehensive analysis of all data encompassing antioxidant activity and phenolic compounds in bee bread. The primary objective was to unveil potential clustering patterns among of bee bread samples based on their geographical origins. According to the results, the Total Phenolic Content (TPC) of bee bread samples ranged from 4.393 to 14.917 mg GAE/g dw, while the Total Flavonoid Content (TFC) exhibited variation within the range of 0.681 to 3.504 mg QE/g dw. p-OH benzoic and p-coumaric acids were detected in all samples. Other phenolic compounds were identified in different proportions among the bee bread samples. This study demonstrated the successful application of the PCA chemometric method to assess the antioxidant activity and phenolic compounds of bee bread samples from various regions of T & uuml;rkiye. The results revealed effective clustering of the bee bread samples based on their geographical origins.
Antioxidant activity of phthalonitrile derivatives bearing different chalcone groups
(Miraç OCAK, 2020) Kamiloğlu, Ayşe Aktaş; Can, Zehra; Çelik, Gonca
This work presents the synthesis of four phthalonitrile compunds (3a-d) bearing chalcone moiety which have biological activities. Phthalonitrile compounds are considerable pioneer in synthesis of novel photoactive phthalocyanine derivatives. The spectroscopic properties (IR, 1H NMR, 13C NMR and mass) and antioxidant activity of the novel (3a and 3b) or previously synthesized (3c and 3d) phthalonitrile compounds were studied. According to the DPPH value, antioxidant activitiy of compound 3b was significantly higher than the counterparts.
Antioxidant, Antimicrobial and Anti-Quorum Sensing Activities of Usnea filipendula and Viscum album
(2019) Yıldız, Sibel; Gürgen, Ayşenur; Kılıç, Ceyhun; Tabbouche, Sana; Kılıç, Ali Osman; Can, Zehra
Many plants contain a variety of bioactive components. Therefore, it is important to know the bioactive properties of plantmaterials in order to be a reference for later researchers. In this study, it was investigated the antioxidant, antimicrobial and anti-quorum sensingactivities of Usnea filipendula and Viscum album’s methanol extracts. To determine the antioxidant properties of the extracts; total phenolic,flavonoid and condensed tannin contents and ferric reducing antioxidant power analyses were performed. The antibacterial potential of plantextracts was tested by agar well diffusion method against Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonasaeruginosa, Salmonella typhimurium, Klebsiella pneumoniae, Proteus mirabilis, Listeria monocytogenes, Candida parapsilosis and Candidaalbicans microorganisms. Anti-quorum sensing activity was investigated on Chromobacterium violaceum bacteria. The highest total phenolicand ferric reducing antioxidant power was determined in U. filipendula extract. This extract inhibited the growth of S. aureus, K. pneumonia andL. monocytogenes microorganisms. The highest flavonoid and condensed tannin was observed in V. album extract. This extract was also able toprevent the growth of K. pneumonia and L. monocytogenes. None of the extracts showed anti-quorum sensing activity.
Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product
(Inst Tecnologia Parana, 2022) Kara, Yakup; Can, Zehra; Kolayl, Sevgi
In this work, a reverse phase high-performance liquid chromatographic method (RP-HPLC) with photo diode array (PDA) at 4 different wavelengths (250, 280, 320 and 360) for the determination of some polyphenols in some bee products (honey, pollen, and propolis) is developed. The analyses were carried out on C18 column (250 mm x 4.6 mm, 5 mu m; GL Sciences), and the gradient program was used with a mobile phase A reservoir with 70% acetonitrile and the B reservoir with 2% acetic acid. The HPLC method was founded that limit of detection was in the range 0.022-0.0908 mu g/mL; the limit of quantification was in the range 0.074-0.3027 mu g/mL, all calibration curves were linear R-2>0.995 within the range, the recovery range was 91.43- 111.37% for 10 ppm and 98.44-101.68% for 40 ppm and relative error levels 0.0330-0.0290 respectively. The developed method was applied to some bee products available on the Turkey market. The study aimed at phenolic profiles of the bee products extracts were revealed by using 25 phenolic standards. The proposed method was optimized, quickly, and simple validated by evaluating the linear range, the limits of detection and quantification, the accuracy, the precious, the repeatability, and recoveries suitable for the phenolic analysis. It is concluded that evaluated and the quantitative determination of the bee products can be made quickly and reliably with the optimized method.
Bee bread from Anatolia: its chemical composition, phenolic and aromatic profiles, and antioxidant properties
(Taylor & Francis Ltd, 2024) Kolayli, Sevgi; Okumus Yukunc, Gulsah; Kara, Yakup; Demir Kanbur, Esra; Can, Zehra; Ozkok, Asli; Tanugur Samanci, Asli Elif
This study investigated the botanical origin, chemical composition, phenolic and volatile aromatic profiles, and antioxidant activities of 11 bee bread samples from different regions of Anatolia, Turkey. The bee bread samples contained high amounts of proteins (19.61 g/100 g) and lipids (6.43 g/100 g). The bee breads were determined to have a rich mineral content. The antioxidant potential of the bee breads was predicted using total phenolic content (TPC), total flavonoid content (TFC), total condensed tannin (TCT) content, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The TPC of the bee breads ranged between 2.041 +/- 0.170 and 3.224 +/- 0.006 mg GAE/g, while the TFC ranged from 0.323 +/- 0.004 to 1.903 +/- 0.017 mg QE/g. The FRAP assay showed antioxidant activity ranging from 17.778 +/- 0.207 to 49.752 +/- 0.856 mu mol FeSO4.7H2O/g, and the DPPH values were between 1.054 +/- 0.009 and 4.366 +/- 0.014 SC50 mg/mL. Using 25 standards, RP-HPLC-PDA quantified the composition of the phenolic compounds, attributed to their antioxidant activity. The highest concentrations were detected for t-cinnamic acid, rutin, and p-coumaric acid. One hundred nineteen volatile aromatic compounds were determined in the bee breads by solid-phase microextraction coupled with gas chromatography and mass spectrometry (SPME-GC-MS). The obtained values suggest that bee breads could serve as a potential source of nutrients and bioactive compounds for value-added food supplements and functional foods.
Characteristic Properties of Spurge (Euphorbia macroclada Boiss.) Honey in Diyarbakır Region
(Oktay YILDIZ, 2020) Kara, Yakup; Birinci, Ceren; Birinci, Esra; Can, Zehra
Sütleğen otu veya bitkisi (Euphorbia macroclada Bois.) ülkemizde yaygın olarak yetişen otsu bir bitki tür olup, her sütleğen otu bal nektar üretmez. Halk arasında sütlüce otu olarak da bilinen bu zehirli bitki, zehirli olmayan nektarlara sahiptir. Ülkemizde en yoğun olarak Güney Doğu Anadolu bölgesinde özellikle Diyarbakır ve Adıyaman bölgelerinde Ağustos ve Eylül aylarında üretilen sütleğen balı (haşul balı) apiterapik bal olarak çok değişik amaçlar için kullanılmaktadır. Mercimek ve nohut hasatından boş kalan tarlalarda yetişen sütleğen çiçeklerinden üretilmektedir. Sütleğen nektarına göre amber renginden oldukça koyu renge kadar değişen renk ve tatlardaki bu bal, oldukça vizkoz, kolay kristalize olmayan, hafif eksimsi bir tada ve aromaya sahiptir. Yapılan bu çalışmada ile ilk kez sütleğen balının bazı fiziko-kimyasal ve antioksidan özellikleri araştırıldı. Çiçek ballarınna göre daha yüksek Fruktoz/Glukoz oranı (1.4-1.7) ve yüksek fenolik madde miktarı (56-82 mg gallik asit (GAE/100 g) ve yüksek antioksidan kapasiteye ile apiterapötik değeri yüksek ballardan biridir. Ancak, yapılacak daha ileri çalışmalar bu balın gerçek biyolojik aktif değerini ortaya çıkaracaktır.
Characterization of the black cumin (Nigella sativa L.) honey from Turkiye
(Elsevier, 2023) Kemal, Mehmet; Esertas, Ulku Zeynep Ureyen; Kanbur, Esra Demir; Kara, Yakup; Ozcelik, Ali Erdem; Can, Zehra; Kolayli, Sevgi
This case report study investigated the botanical, physicochemical, and phenolic compositions, as well as the antioxidant and antimicrobial properties of varying amounts of black cumin honey (Nigella sativa) from Burdur region of Turkiye. The samples had an average moisture value of 17.23% and a Nigella sativa pollen ratio ranging from 19% to 51%. Electrical conductivity (EC) values ranged from 0.36 to 0.54 mS/cm. The average color values of Hunter (L, a, b) were 43.55 (L*), 32.77 (a*), and 64.52 (b*), respectively. The proline contents were ranged from 875 to 1624 mg/kg. The average F/G ratio and F+G value were 1.43 +/- 0.11 and 68.75 +/- 4.75, respectively. The average of total phenolic (TP) and total flavonoid (TF) contents were 36.87 +/- 7.00 mg GAE/100 g and 5.63 +/- 1.45 mg QUE/100 g, respectively. The antioxidant capacities of the samples were tested using the ferric reducing/antioxidant power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays, with mean values of 179.40 +/- 25.35 mu mol FeSO4 center dot 7H(2)O/100 g and 46.07 +/- 12.87 mg/mL, respectively. The phenolic composition of the samples were tested using the HPLC-PDA method with 25 phenolic standards. Gallic acid, p-OH benzoic acid, t-cinnamic acid, p-coumaric acid, chrysin, and pinocembrin were determined as common components. The samples exhibited high antimicrobial activities against Staphylococcus aureus, Escherichia coli, Chromobacterium violaceum, Bacillus cereus, Klebsiella pneumoniae, and Acinetobacter haemolyticus, but did not affect Candida albicans, Candida parapilopsis, or Mycobacterium smegmatis.
Chemical and bioactive potential of the nests of Polistes nimpha, Polistes dominula, and Vespa crabro (Hymenoptera: Vespidae)
(Pamukkale University, 2025) Ertürk, Ömer; Can, Zehra; Yaman, Mustafa
This work was accomplished to establish the chemical components and bioactivity potential of the nest materials of Polistes nimpha (Christ), Polistes dominula (Christ), and Vespa crabro (L.). The biological and chemical compounds of materials and their molecular functionalities were detected using FRAP (ferric reducing antioxidant power) method, DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method, and Energy Dispersive X-Ray Analysis. Finally, the bioactivity potentials of nest extracts were assayed and phenolic components were determined. C, N, O, Na, Ca, K, Mg, Al, Si are the elemental components of the nest materials. All nest extracts of three species had high biological activity against nine bacteria and one fungus causing common infections. The maximum antibacterial and antifungal activity was seen when gram-negative Pseudomonas aeruginosa, gram-positive Bacillus cereus, and gram-positive Candida albicans were exposed to ethanol extracts of nest samples in 25 ?L. High antioxidant activity can be found in the nest extracts. These extracts might help researchers find novel antifungal and antibacterial substances. © 2025 Pamukkale University. All rights reserved.
Comparative Study of Some Commercial Propolis Extract with New Prepared Ethanolic Propolis Extract
(Arıcılık Araştırma Enstitüsü Müdürlüğü, 2020) Kolaylı, Sevgi; Kara, Yakup; Can, Zehra
In this study, quality parameters of some commercially sold in Turkey propolis extracts were compared with freshly prepared ethanolic propolis extract (70%). The pH, total phenolic substance, total flavonoid and total antioxidant capacity values of 11 different commercial samples, including aqueous, ethanolic, water?based and vegetable oil, were compared. As a result, it was determined that ethanolic samples had the highest activity and the activity of propolis with olive oil was very low. It was found that products with high total polyphenol content (TP) had high total antioxidant values. It was also determined that a standard method was not used for the preparation of commercial propolis samples and that the TP values differ greatly.
Comparison of Phenolic Profile and Antioxidant Properties of Pulp and Seeds of Two Different Grapes Types (Vitis vinifera L. and Vitis labrusca L.) Grown in Anatolia: The Amount of Resveratrol of Grape Samples
(Springer Science and Business Media Deutschland GmbH, 2023) Kavgacı, Merve; Yukunc, Gulsah Okumus; Keskin, Merve; Can, Zehra; Kolaylı, Sevgi
Background: Grape is the most widely cultivated fruit all over the World and it is both food and a good source of antioxidants. The fruit is rich in phytochemicals and its seed is especially a source for resveratrol, a stilbene that shows health promoting effect. Objective: In this study, phenolic profile and antioxidant properties of eight grape cultivars with two species cultivated in Turkey were compared. Isabella, Razaki, Alphonse Lavalleé, Red Globe and Emir cultivars were used. Methods: Total phenolic substance (TPS), total flavonoid substance (TFS), total antioxidant capacity (FRAP) and phenolic compositions of the pulp and the seeds were determined. Phenolic composition of grapes were found by using HPLC-UV. Results: The results showed that total phenolic substances and total antioxidant capacities were richer in the seeds than in the pulps. Catechin and epicatechin are major phenolic compounds in the grape seeds, and chrysin, pinocembrin, resveratrol, luteolin and caffeic acid phenethyl ester (CAPE) were detected in the pulp as major. Resveratrol varied from 4.50 to 101.89 mg/100 g, while was equally in both the seed and pulp. It was found that all grape samples were rich in phenolic compounds and had antioxidant activity. Conclusion: It was clear that antioxidant capacity and phenolic profile of samples changes according to grape species and cultivars. © 2023, The Tunisian Chemical Society and Springer Nature Switzerland AG.
Design, synthesis, antioxidant and anticholinesterase activities of novel isonicotinic hydrazide-hydrazone derivatives
(Elsevier, 2023) Aslanhan, Ozlem; Kalay, Erbay; Tokali, Feyzi Sinan; Can, Zehra; Sahin, Engin
The design and synthesis of hydrazone derivatives are increasing in popularity day by day due to the significant biological activities of this scaffold. In the present study, twelve novel isonicotinic hydrazide-hydrazone analogues were synthesized by the condensation reaction of isonicotinic hydrazide with ben-zaldehyde possessing sulfonate moiety. The structures of the novel compounds have been characterized in detail using spectroscopic techniques. All compounds have shown inhibitory effects against the AChE en-zyme at rates ranging from 21.00 to 59.48%. Among them, compound 5 has exhibited the best inhibitory effect of 59.48% against AChE at a concentration of 0.1 mM. Furthermore, to determine how effective the novel compounds are as antioxidants, FRAP and DPPH studies were also carried out. FRAP values in compounds 1-12 were found to range from 26.989-3415.556 mu mol FeSO4.7H2O/mg. They also displayed moderate antioxidant potential in the range of SC50= 0.03-87.32 mg/mL compared with the control Trolox (SC50 = 0.004) in DPPH radical scavenging activities. It was seen that the AChE inhibition percentages of the compounds were in the range of 23.04-58.10% at 0.1 mM concentration. This is the first research on the synthesis, antioxidant and enzyme inhibition properties of these compounds. (c) 2023 Elsevier B.V. All rights reserved.
DETERMINATION OF ANTI-UREASE ACTIVITY OF PROPOLIS FROM MARMARA REGION OF TURKEY
(Bursa Uludag University, 2022) Can, Zehra; Kara, Yakup; Kolayli, Sevgi; Çakmak, Ibrahim
Propolis is a natural product collected by honeybees from plants especially flowers and buds by mixing with wax and resin and used for many purposes in hive. It is known that propolis has been used in the treatment of various diseases in traditional medicine for many years and has biological activities such as antioxidant, antimicrobial, antiulcer, antitumor, anti-inflammatory. There is increasing interest in alternative approaches to inhibit Helicobacter pylori (H. pylori) and thereby treat many gastric diseases. This study investigated various properties of raw propolis samples from the Marmara region of Turkey. Anti-urease activity was investigated in propolis samples. The anti-urease activity of IC50 values ranged from 1.110 to 5.870 mg/mL. As a result, it can be said that propolis extract is a good inhibitor that can be used in the treatment of H. pylori to improve human health. © 2022 Uludag Aricilik Dergisi. All rights reserved.
DETERMINATION OF ANTIOXIDANT, ANTIMICROBIAL ACTIVITIES, PHENOLIC COMPOUNDS OF WILD ROSA L. SPECIES BAYBURT, TURKEY
(Parlar Scientific Publications (P S P), 2019) Gidik, Betul; Akar, Zeynep; Can, Zehra; Sefali, Abdurrahman; Erturk, Omer
Five different rosehip (Rosa L.) species including, Rosa canina, Rosa iberica Stev, Rosa villosa, Rosa beggeriana Schrenk and same kind of but different location Rosa spinosissima grown in Bayburt from Turkey are studied. The wild species of Rosa L. were collected from Rocks and Steps, Field and garden borders. Morphological parameters were measured as fruit and seed weights and fruit and seed size. Ten fruits were chosen randomly for the measurement and take the average of them to determine the weights and size. In our study was determined antioxidant activity, antimicrobial activity and phenolic compounds. As antioxidant activity; total phenolic total flavonoid, condense tannin contents, CUPRAC, FRAP, ABTS. Nineteen standard of phenolic compositions of fruits and seeds were determined. Antioxidant, antimicrobial activities of the extracts were found high levels in rose hip species fruit than seed. Catechin, rutin protocatequic acid and CAPE were principal for all rose hip species fruit but, was not deteceted CAPE Kop Mountain, Yukari Kop Village Rosa spinosissima species. This suggests that the difference in location affects the phenolic content. Pearson Correlation analyze has done with the morphological parameters, GPS data, antioxidant activity and phenolic compounds by using the SPSS software programme version 25.0.
Determination of cytotoxic, anti-acetylcholinesterase and antioxidant activity of some medicinal Artemisia spp.
(2023) Can, Zehra; Demir, Elif Ayazoglu; Akar, Zeynep; Kara, Yakup; Gıdık, Betül
The study aims to investigate the antioxidant activities, phenolic compounds, acetylcholinesterase enzyme inhibition and cytotoxic effects of two different Artemisia spp. (Artemisia dracunculus L. and Artemisia dracunculoides Pursh) cultivated in Türkiye organically, for the first time. The total phenolic and total flavonoid contents of the plants were determined spectrophotometrically while antioxidant activity DPPH•, CUPRAC, and FRAP was determined using the colorimetric method. And also, acetylcholinesterase in vitro enzyme inhibition activity and anti-cancer activity against human melanoma (VMM917, CRL-3232), lung carcinoma (A549, CCL-185), and normal human fibroblast (hGF, PCS-201-018) cells were studied. Total phenolic (225-324 ?g GAE/mL extract) and total flavonoid contents (0.066-0.085 ?g QE/mL extract), antioxidant activity Color Scavenging Concentration 50% (DPPH• (CSC50: 1.371-1.655 mg/mL), CUPRAC (0.246-0.344 ?M CTEAC) and FRAP (462.133-726.661 ?M CTEAC)). A. dracunculus and A. dracunculoides extracts inhibited 40.09 ± 0.65%, and 39.48 ± 3.68% of acetylcholinesterase activity. It was determined that demonstrated the selective effect of A. dracunculus and A. dracunculoides on the cytotoxicity of A549 and hGF cells. According to our results, both tarragon plant species, it may be said that it is not only consumed as food but also used for therapeutic purposes will be beneficial for health.
Determination of cytotoxic, anti-acetylcholinesterase and antioxidant activity of some medicinal Artemisia spp.
(2023) Can, Zehra; Demir, Elif Ayazoglu; Akar, Zeynep; Kara, Yakup; Gıdık, Betül
The study aims to investigate the antioxidant activities, phenolic compounds, acetylcholinesterase enzyme inhibition and cytotoxic effects of two different Artemisia spp. (Artemisia dracunculus L. and Artemisia dracunculoides Pursh) cultivated in Türkiye organically, for the first time. The total phenolic and total flavonoid contents of the plants were determined spectrophotometrically while antioxidant activity DPPH•, CUPRAC, and FRAP was determined using the colorimetric method. And also, acetylcholinesterase in vitro enzyme inhibition activity and anti-cancer activity against human melanoma (VMM917, CRL-3232), lung carcinoma (A549, CCL-185), and normal human fibroblast (hGF, PCS-201-018) cells were studied. Total phenolic (225-324 ?g GAE/mL extract) and total flavonoid contents (0.066-0.085 ?g QE/mL extract), antioxidant activity Color Scavenging Concentration 50% (DPPH• (CSC50: 1.371-1.655 mg/mL), CUPRAC (0.246-0.344 ?M CTEAC) and FRAP (462.133-726.661 ?M CTEAC)). A. dracunculus and A. dracunculoides extracts inhibited 40.09 ± 0.65%, and 39.48 ± 3.68% of acetylcholinesterase activity. It was determined that demonstrated the selective effect of A. dracunculus and A. dracunculoides on the cytotoxicity of A549 and hGF cells. According to our results, both tarragon plant species, it may be said that it is not only consumed as food but also used for therapeutic purposes will be beneficial for health.
DETERMINATION OF IN -VITRO ANTIOXIDANT, ANTI-UREASE, ANTI-HYALURONIDASE ACTIVITIES BY PHENOLIC RICH BEE PRODUCTS FROM DIFFERENT REGION OF TURKEY
(Parlar Scientific Publications (P S P), 2018) Can, Zehra
In this study, enzyme inhibition of urease and hyaluronidase are clinically important functions were examined by different authenticity bee products. Different floral honey, pollen and propolis samples were analyzed in terms of total phenolic compounds (TPC), total flavonoid contents (TFC), ferric reducing antioxidant capacity (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenyl-l-picryhydrazyl (DPPH) free radical scavenging activities as antioxidant determinants. For the determination of the phenolic composition of the products, RP-UV-HPLC was used. TPC of the honey, pollen and propolis samples were ranged from 17 to 95 and 770 to 989 mg of gallic acid (GAE)/100g, 135-258 mg of GAE/g, and TFC was 1 to 10 mgQE/100g, and 78 to 99 mgQE/100 g, and 9 to 10 mg of QE/g, respectively. Among the bee products, propolis samples were showed the highest phenolic componds, that contained different quantity of protocatequic acid, catechin, caffeic acid, p-coumaric acid, p- hydroxybenzoic acid, ferulic acid and rutin. The urease inhibition vales (IC50) were changed from 7.02 to 33.25 mg/mL, 5.00 to 8.78 and 0.16 to 1.98 mg/mL in the honey, pollen and propolis samples, respectively. The hyaluronidase inhibition values (IC50) were changed from 0.02 to 0.46 g/mL, 0.02 to 0.26 g/mL and 3.26 to 9.21 mu g/mL in the honey, pollen and propolis samples, respectively. Honey, pollen and propolis have a significant inhibition on the enzymes, the inhibition may be due to secondary metabolites of phenolic compounds and the mechanism of the inhibition should be studied in detailed.

Yazar "Can, Zehra" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri