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    Antagonistic activities of lactic acid bacteria isolated from Pastirma
    (Elsevier Science Bv, 2016) Cinar, Kubra; Kaya, Mukerrem; Kaban, Guzin
    [Abstract Not Available]
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    Assessment of technological attributes of autochthonous starter cultures in Turkish dry fermented sausage (sucuk)
    (Wiley, 2022) Kaban, Guzin; Sallan, Selen; Topcu, Kubra Cinar; Borekci, Bilge Sayin; Kaya, Mukerrem
    The study aims to evaluate the technological properties of autochthonous strains (Lactobacillus sakei S15, Lactobacillus plantarum S24, L. plantarum S91, Pediococcus pentosaceus S128b and Staphylococcus carnosus G109) in Turkish dry fermented sausage (sucuk). After 24 h of fermentaPtion, all lactic acid bacteria strains reduced the pH to below 5.0, while the pH in the control group was above 5.3. The number of lactic acid bacteria strains reached 10(8)-10(9) cfu g(-1) during fermentation. Staphylococcus carnosus G109 remained at the inoculation level of 10(6) cfu g(-1) during ripening. Lactobacillus sakei S15 as mono-culture showed higher TBARS values compared to other strains. The control group had the lowest L* value and autochthonous strains caused no significant difference for a* value. According to principal component analysis results, most volatile compounds were positively correlated with the group containing only L. sakei S15.
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    Catalase positive cocci and volatile compounds in fermented sausages
    (Elsevier Science Bv, 2014) Kamiloglu, Aybike; Sayin, Bilge; Kaban, Guzin; Kaya, Mukerrem
    [Abstract Not Available]
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    Genotypic Identification of Lactic Acid Bacteria in Pastirma Produced w h Different Curing Processes
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2019) Cinar, Kubra; Fettahoglu, Kubra; Kaban, Guzin
    The lactic acid bacteria isolated from pastirma, produced under controlled conditions using two different curing temperatures (4 degrees C or 10 degrees C) and two different curing agents (150 mg/kg sodium nitrite or 300 mg/kg potassium nitrate), were subjected to genotypic (16S rRNA sequecing) identification. According to the identification results, 68 of 87 isolates (78.16%) was identified as Pediococcus pentosaceus. This species was followed by P. acidilactici (14.94%), Lactobacillus sakei (4.60%) and L plantarum (2.30%), respectively. P pentosaceus was dominant species in all curing applications (4 degrees C/nitrate or nitrite or 10 degrees C/nitrate or nitrite). Another species determined in all groups was P. acidilactici. While L. plantarum was only isolated from samples produced with nitrate (4 degrees C or 10 degrees C), L sakei was isolated from samples produced with nitrite (4 degrees C or 10 degrees C). The effect of the curing agent on the biodiversity of lactic acid bacteria in pastirma was more effective than the curing temperature.
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    Identification and characterization of lactic acid bacteria isolated from sucuk, a traditional Turkish dry-fermented sausage
    (Elsevier Science Bv, 2018) Cinar, Kubra; Kaban, Guzin; Borekci, Bilge Sayin; Gulluce, Medine; Karadayi, Mehmet; Kaya, Mukerrem
    [Abstract Not Available]
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    Plasma polymerized linalool (ppLin): an antimicrobial and biocompatible coating
    (Tubitak Scientific & Technological Research Council Turkey, 2019) Cakmak, Kenan; Bayram, Ozkan; Solak, Kubra; Kaban, Guzin; Simsek, Onder; Mavi, Ahmet
    Bacterial infections in medical devices and drug resistance of bacteria can cause chaos in the world due to loss of lives in addition to the cost of device revisions, quarantine, disinfection of infected areas, and patient treatment. Antibacterial coatings of essential oils on medical devices can prevent bacterial attachment and reduce costs. Linalool is an antibacterial constituent of essential oils. Herein, we examine for the first time the fabrication and characterization of radio frequency (RF) plasma polymerized hydrophilic thin films from linalool (ppLin) by varying deposition parameters (RF power and deposition time) and the behavior of ppLin with two bacteria (Escherichia coli and Staphylococcus aureus) commonly related to microbial contamination of medical devices. While a dramatic reduction in E. coli and S. aureus attachment was observed on ppLin films, their hydrophilic surface was also bactericidal to S. aureus. Additionally, ppLin films were shown to be adherent and noncytotoxic to human fibroblast cells. ppLin can be potentially integrated into medical and other clinical devices as a promising low-cost biocompatible antimicrobial coating.
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    Probiotic meat products
    (Elsevier Science Bv, 2014) Sayin, Bilge; Kamiloglu, Aybike; Kaya, Mukerrem; Kaban, Guzin
    [Abstract Not Available]
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    Probiotic properties of lactic acid bacteria strains isolated from pastirma
    (Elsevier, 2020) Topcu, Kubra Cinar; Kaya, Mukerrem; Kaban, Guzin
    In this study, the probiotic properties of 80 lactic acid bacteria (LAB) strains isolated from pastirma, a dry-cured meat product, were investigated. For this purpose, strains were investigated for their resistance to bile salt and hydrophobicity abilities. In 6 strains selected as a result of these analyses, development at high salt concentrations, auto-aggregation and co-aggregation, resistance to low pH, and adhesion analyses were performed. The growth percentage of all strains decreased with increasing bile salt concentration at both 25 degrees C and 37 degrees C. P. pentosaceus K7, K41, K44, K51 and K81 and P. acidilactici K99 gave the highest values in terms of hydrophobicity ability. The highest auto-aggregation and the highest co-aggregation were found in P. pentosaceus K41 (17.62%) and P. pentosaceus K44 strain (26.23%), respectively. These six strains showed tolerance to high acidity even at pH 2. The strains significantly maintained their viability in the simulated gastric and intestinal fluids. Furthermore, the strains had low adhesion capacity in Caco-2 and HT-29 cell lines and generally showed resistance under gastrointestinal system conditions. As a result, P. pentosaceus K44, P. acidilactici K99 and P. pentosaceus K41 could be considered as probiotic strains.
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    Technological properties of autochthonous Lactobacillus plantarum strains isolated from sucuk (Turkish dry-fermented sausage)
    (Springer, 2020) Kamiloglu, Aybike; Kaban, Guzin; Kaya, Mukerrem
    Purpose Five Lactobacillus strains isolated from sucuk (Turkish dry-fermented sausage) were studied for their genetic and technological properties. Methods For genotypic identification, strains 16S rRNA gene sequences were used. To determine the antimicrobial activity of strains, seven foodborne pathogens were tested. Strains technological properties were characterized. Results These strains were identified as Lactobacillus plantarum by 16S rRNA gene sequence analysis and the phylogenetic tree obtained by neighbor-joining method allowed grouping of these strains into three subgroups. L. plantarum strains showed antagonistic activities against Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, and Micrococcus luteus strains. PCR assay, using specific primers, showed the presence of bacteriocin (plantaricin) encoding genes in all L. plantarum strains tested. Antimicrobial metabolite production of these strains started at log phase and reached the maximum level at the end of the stationary phase. Regarding their technological properties, better growth was observed at 25 degrees C compared with 15 degrees C and 45 degrees C. The isolates which grown well within the pH scale pH 4.5-6.5 range additionally showed a decent growth at 6.5% salt concentration. It has been found that strains do not exhibit lipolytic and proteolytic activities nor have lysine, ornithine, and arginine decarboxylase activity. On the other hand, one strain showed weak nitrate reductase activity, and four strains produced acetoin from glucose. In addition, all strains were DL-lactic acid producers. Consequently, L. plantarum strains isolated exhibited some biochemical properties required for a starter culture in sucuk and similar products. Conclusions All identified strains may be a protective culture in the production of fermented meat products. In particular, L. plantarum S51 was distinguished from other isolates due to the inability to form acetoin from glucose. Further work will be needed to characterize L. plantarum strains as starter culture.