Correlation between the DNA methylation and gene expression of IGFBP5 in breast cancer

Basit Öğe Kaydı
dc.authorid55771646300
dc.authorid55876457500
dc.authorid56960629200
dc.authorid56448719400
dc.authorid7801655696
dc.authorid57192895317
dc.authorid7102340078
dc.authorid23481935200
dc.authorid7005139386
dc.authorid57195257114
dc.contributor.authorKarabulut S.
dc.contributor.authorKaya Z.
dc.contributor.authorAmuran G.G.
dc.contributor.authorPeker I.
dc.contributor.authorÖzmen T.
dc.contributor.authorGulluolu B.M.
dc.contributor.authorKaya H.
dc.contributor.authorErzik C.
dc.contributor.authorOzer A.
dc.contributor.authorAkkiprik M.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:43:45Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:43:45Z
dc.date.issued2016
dc.departmentBayburt Üniversitesien_US
dc.description.abstractBACKGROUND: The insulin-like growth factor binding protein5 (IGFBP5) is often dysregulated in human cancers and considered neither a tumor suppressor nor an oncogene. OBJECTIVE: We aim to examine the reason of the changeable gene regulation of IGFBP5 in the case of methylation in breast cancer. METHODS: We used methyl-specific polymerase (MSP) chain reaction to detect CpG methylation of IGFBP5 promoter and exon-I in breast cancer and adjacent tissues. Gene expression is evaluated by quantative polymerase chain reaction (qPCR). RESULTS: IGFBP5 methylation was detected in 24 of 58 (41%) and 54 of 56 (96.5%) promoter and exon-I site respectively in tumor tissues. In adjacent tissues 17 of 58 (29%) and 53 of 56 (96.5%) was methylated. IGFBP5 expression was higher estrogene receptor (ER)(+) than ER(-) patients (p = 0:0549). Beside, we found a positive correlation between the expression of IGFBP5 and G2 tumor grade (p = 0:0131). However, no correlation was observed between IGFBP5 expression and age, menopause or the presence of lymph node metastasis (p > 0:05). CONCLUSIONS: In summary, our results showed that IGFBP5 promoter and exon-I methylation did not have any differences between tumor and adjacent tissues so that IGFBP5 methylation did not change IGFBP5 gene regulation in breast cancer. This is the first study investigating the IGFBP5 gene methylation in breast cancer. © 2016 - IOS Press and the authors.en_US
dc.identifier.doi10.3233/BD-160234
dc.identifier.endpage131
dc.identifier.issn0888-6008
dc.identifier.issue4
dc.identifier.pmid27612043en_US
dc.identifier.scopus2-s2.0-85008616079en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage123
dc.identifier.urihttps://dx.doi.org/10.3233/BD-160234
dc.identifier.urihttps://hdl.handle.net/20.500.12403/668
dc.identifier.volume36
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIOS Press
dc.relation.ispartofBreast Diseaseen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbreast cancer
dc.subjectIGFBP5
dc.subjectmethylation
dc.subjectMSP
dc.subjectpromoter
dc.subjectbeta actin
dc.subjectepidermal growth factor receptor 2
dc.subjectestrogen receptor
dc.subjectKi 67 antigen
dc.subjectsomatomedin binding protein 5
dc.subjectepidermal growth factor receptor 2
dc.subjectERBB2 protein, human
dc.subjectestrogen receptor
dc.subjectmessenger RNA
dc.subjectprogesterone receptor
dc.subjectsomatomedin binding protein 5
dc.subjectadult
dc.subjectage distribution
dc.subjectaged
dc.subjectArticle
dc.subjectbinding site
dc.subjectbreast cancer
dc.subjectcancer grading
dc.subjectcancer prognosis
dc.subjectcontrolled study
dc.subjectCpG island
dc.subjectDNA methylation
dc.subjectfemale
dc.subjectgene expression regulation
dc.subjectgene sequence
dc.subjecthistopathology
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectlymph node metastasis
dc.subjectmajor clinical study
dc.subjectpolymerase chain reaction
dc.subjectpostmenopause
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectprotein expression
dc.subjecttranscription initiation site
dc.subjectbreast tumor
dc.subjectexon
dc.subjectgene expression
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmiddle aged
dc.subjectpathology
dc.subjectAdult
dc.subjectAged
dc.subjectBreast Neoplasms
dc.subjectCpG Islands
dc.subjectDNA Methylation
dc.subjectExons
dc.subjectFemale
dc.subjectGene Expression
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHumans
dc.subjectInsulin-Like Growth Factor Binding Protein 5
dc.subjectMiddle Aged
dc.subjectNeoplasm Grading
dc.subjectPolymerase Chain Reaction
dc.subjectPromoter Regions, Genetic
dc.subjectReceptor, ErbB-2
dc.subjectReceptors, Estrogen
dc.subjectReceptors, Progesterone
dc.subjectRNA, Messenger
dc.subjectbreast cancer
dc.subjectIGFBP5
dc.subjectmethylation
dc.subjectMSP
dc.subjectpromoter
dc.subjectbeta actin
dc.subjectepidermal growth factor receptor 2
dc.subjectestrogen receptor
dc.subjectKi 67 antigen
dc.subjectsomatomedin binding protein 5
dc.subjectepidermal growth factor receptor 2
dc.subjectERBB2 protein, human
dc.subjectestrogen receptor
dc.subjectmessenger RNA
dc.subjectprogesterone receptor
dc.subjectsomatomedin binding protein 5
dc.subjectadult
dc.subjectage distribution
dc.subjectaged
dc.subjectArticle
dc.subjectbinding site
dc.subjectbreast cancer
dc.subjectcancer grading
dc.subjectcancer prognosis
dc.subjectcontrolled study
dc.subjectCpG island
dc.subjectDNA methylation
dc.subjectfemale
dc.subjectgene expression regulation
dc.subjectgene sequence
dc.subjecthistopathology
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectlymph node metastasis
dc.subjectmajor clinical study
dc.subjectpolymerase chain reaction
dc.subjectpostmenopause
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectprotein expression
dc.subjecttranscription initiation site
dc.subjectbreast tumor
dc.subjectexon
dc.subjectgene expression
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmiddle aged
dc.subjectpathology
dc.subjectAdult
dc.subjectAged
dc.subjectBreast Neoplasms
dc.subjectCpG Islands
dc.subjectDNA Methylation
dc.subjectExons
dc.subjectFemale
dc.subjectGene Expression
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHumans
dc.subjectInsulin-Like Growth Factor Binding Protein 5
dc.subjectMiddle Aged
dc.subjectNeoplasm Grading
dc.subjectPolymerase Chain Reaction
dc.subjectPromoter Regions, Genetic
dc.subjectReceptor, ErbB-2
dc.subjectReceptors, Estrogen
dc.subjectReceptors, Progesterone
dc.subjectRNA, Messenger
dc.titleCorrelation between the DNA methylation and gene expression of IGFBP5 in breast canceren_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu