Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: An in vitro study

Küçük Resim Yok

Tarih

2014

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Asian Pacific Tropical Biomedicine Press

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Objective: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). Methods: hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. Results: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. Conclusions: Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro. © 2014 by the Asian Pacific Journal of Tropical Biomedicine.

Açıklama

Anahtar Kelimeler

Cefoperazone sodium, Inhibition, Meropenem trihydrate, Paraoxonase, Piperacillin sodium, aryldialkylphosphatase 1, cefoperazone, meropenem, piperacillin, anion exchange chromatography, Article, binding affinity, competitive inhibition, concentration (parameters), controlled study, drug protein binding, enzyme activity, enzyme inhibition, enzyme purification, gel filtration chromatography, human, IC50, in vitro study, Cefoperazone sodium, Inhibition, Meropenem trihydrate, Paraoxonase, Piperacillin sodium, aryldialkylphosphatase 1, cefoperazone, meropenem, piperacillin, anion exchange chromatography, Article, binding affinity, competitive inhibition, concentration (parameters), controlled study, drug protein binding, enzyme activity, enzyme inhibition, enzyme purification, gel filtration chromatography, human, IC50, in vitro study

Kaynak

Asian Pacific Journal of Tropical Biomedicine

WoS Q Değeri

Scopus Q Değeri

N/A

Cilt

4

Sayı

8

Künye