Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: An in vitro study
Küçük Resim Yok
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Asian Pacific Tropical Biomedicine Press
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). Methods: hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. Results: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. Conclusions: Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro. © 2014 by the Asian Pacific Journal of Tropical Biomedicine.
Açıklama
Anahtar Kelimeler
Cefoperazone sodium, Inhibition, Meropenem trihydrate, Paraoxonase, Piperacillin sodium, aryldialkylphosphatase 1, cefoperazone, meropenem, piperacillin, anion exchange chromatography, Article, binding affinity, competitive inhibition, concentration (parameters), controlled study, drug protein binding, enzyme activity, enzyme inhibition, enzyme purification, gel filtration chromatography, human, IC50, in vitro study, Cefoperazone sodium, Inhibition, Meropenem trihydrate, Paraoxonase, Piperacillin sodium, aryldialkylphosphatase 1, cefoperazone, meropenem, piperacillin, anion exchange chromatography, Article, binding affinity, competitive inhibition, concentration (parameters), controlled study, drug protein binding, enzyme activity, enzyme inhibition, enzyme purification, gel filtration chromatography, human, IC50, in vitro study
Kaynak
Asian Pacific Journal of Tropical Biomedicine
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
4
Sayı
8
