Purification and characterisation of laccase from lactarius volemus and its application in removal of phenolic compounds from fruit juice

Basit Öğe Kaydı
dc.authorid6506121388
dc.authorid8064145700
dc.contributor.authorNadaroglu H.
dc.contributor.authorTasgin E.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:44:31Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:44:31Z
dc.date.issued2013
dc.departmentBayburt Üniversitesien_US
dc.description.abstractLaccases are multicopper oxidases (EC 1.10.3.2.), widely distributed in fungi, higher plants and bacteria. In this research, a laccase from Lactarius volemus (Russulaceae) was purified by 73.08 fold using precipitate of saturation (NH4)2SO4, DEAE-cellulose and Sephacryl S200. It was shown that purified enzyme was homogeneous in term of SDS-PAGE with molecular mass estimated of 30 kDa. The pH was optimal at 4.0, enzyme was stable at pH 3.0-5.0 and optimal temperature was at 50°C. Cu2+ and Fe2+ were activated to the purified laccase enzyme, and it was partially inhibited by 5 mM concentration of some metal salts and EDTA, urea, SDS, L-cysteine, dithioeritritol and ?-mercaptoethanol. In addition, it was investigated whether the purified and characterized laccase enzyme would remove the phenolic compound of some fruit juices which are responsible for haze formation and browning during storage. Their application led to reduction of total phenolic compounds for apple, apricot, cherry and grape juice. As a result, the effect of these enzymatic treatments on total phenol content and clarity of the juice were evaluated using a central composite design. This laccase enzyme had potential application in food processing.en_US
dc.identifier.endpage114
dc.identifier.issn1459-0255
dc.identifier.issue03.Apr
dc.identifier.scopus2-s2.0-84886283813en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage109
dc.identifier.urihttps://hdl.handle.net/20.500.12403/874
dc.identifier.volume11
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWFL Publisher Ltd.
dc.relation.ispartofJournal of Food, Agriculture and Environmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCharacterization
dc.subjectFruit juice
dc.subjectLaccase
dc.subjectLactarius volemus (Russulaceae)
dc.subjectPurification
dc.subjectbarium
dc.subjectcadmium
dc.subjectcalcium
dc.subjectcopper
dc.subjectcysteine
dc.subjectdithiothreitol
dc.subjectedetic acid
dc.subjectiron
dc.subjectlaccase
dc.subjectmagnesium
dc.subjectmercaptoethanol
dc.subjectmercury
dc.subjectmetal ion
dc.subjectnickel
dc.subjectphenol
dc.subjecturea
dc.subjectzinc
dc.subjectanion exchange chromatography
dc.subjectarticle
dc.subjectenzyme activity
dc.subjectenzyme analysis
dc.subjectenzyme purification
dc.subjectfood processing
dc.subjectfruit juice
dc.subjectgel filtration
dc.subjectLactarius volemus
dc.subjectmushroom
dc.subjectnonhuman
dc.subjectpH
dc.subjectpolyacrylamide gel electrophoresis
dc.subjecttemperature
dc.subjectEmbryophyta
dc.subjectFungi
dc.subjectLactarius volemus
dc.subjectMalus x domestica
dc.subjectPrunus armeniaca
dc.subjectRussulaceae
dc.subjectVitaceae
dc.subjectCharacterization
dc.subjectFruit juice
dc.subjectLaccase
dc.subjectLactarius volemus (Russulaceae)
dc.subjectPurification
dc.subjectbarium
dc.subjectcadmium
dc.subjectcalcium
dc.subjectcopper
dc.subjectcysteine
dc.subjectdithiothreitol
dc.subjectedetic acid
dc.subjectiron
dc.subjectlaccase
dc.subjectmagnesium
dc.subjectmercaptoethanol
dc.subjectmercury
dc.subjectmetal ion
dc.subjectnickel
dc.subjectphenol
dc.subjecturea
dc.subjectzinc
dc.subjectanion exchange chromatography
dc.subjectarticle
dc.subjectenzyme activity
dc.subjectenzyme analysis
dc.subjectenzyme purification
dc.subjectfood processing
dc.subjectfruit juice
dc.subjectgel filtration
dc.subjectLactarius volemus
dc.subjectmushroom
dc.subjectnonhuman
dc.subjectpH
dc.subjectpolyacrylamide gel electrophoresis
dc.subjecttemperature
dc.subjectEmbryophyta
dc.subjectFungi
dc.subjectLactarius volemus
dc.subjectMalus x domestica
dc.subjectPrunus armeniaca
dc.subjectRussulaceae
dc.subjectVitaceae
dc.titlePurification and characterisation of laccase from lactarius volemus and its application in removal of phenolic compounds from fruit juiceen_US
dc.typeArticleen_US

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