Cirit atlarında müsabakanın serum irisin ve ampk düzeyleri, bazı kas hasarı, stres belirteçleri ve biyokimyasal parametreler üzerine etkisi
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bayburt Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Cirit, atlı oyuncuların kendilerini zinde tutmak için oynadıkları yiğitlik, cesaret oyunu ve erliğin timsali olarak kabul edilen Türk binicilik sporlarının en eskisi olarak kabul edilen at üstünde oynanan, tarihî ve millî atlı takım sporlarından biridir.Bu kapsamda çalışmada etkisini incelediğimiz hormonlardan birisi olan irisin, iskelet kasında yoğun bulunması ve egzersiz hormonu olarak adlandırılması nedeniyle atların performans seviyesinin belirlenmesinde değerlendirilebileceği bildirilen adipokin ve miyokindir. AMPK, ise hücresel enerji sensörü olarak kabul edilen enerji homeostazında rol oynayan bir enzimdir. Bu araştırma, Bayburt ilinde düzenlenen cirit müsabakasına katılan atlarda müsabakanın (istirahat hali, müsabaka öncesi ve müsabaka sonu) serum irisin, AMPK, IL-6, GRP78, kortizol hormon düzeyleri üzerine etkisi ve bazı biyokimyasal (glukoz, laktat, CK, CK-MB, üre, kreatinin, AST, ALT, ALP, GGT, LDH, Total kolesterol (TK), Trigliserid (TG), VLDL, LDL, HDL)parametrelerle ilişkisinin incelenmesidir. Bu kapsamda araştırmada yaşları 4-10 arasında değişen toplam 14 adet Arap aygırı kullanıldı. Çalışmaya dahil edilen atlardan toplamda iki müsabaka süresince istirahat hali (H0), müsabaka öncesi (H1) ve müsabaka sonu (H2) dönemleri olmak üzere vena jugularis'ten kan örnekleri alındı. Kan serumu örneklerinde irisin, AMPK, IL-6, GRP78, kortizol hormonu düzeyleri ELISA yöntemiyle, biyokimyasal parametreler, Cobas 8000 otoanalizörde spektrofotometrik yöntemle kantitatif olarak çalışıldı. Alınan kan örneklerinden elde edilen serumlar -80 C°'de saklandı. Verilere ait istatistiksel değerlendirmede IBM SPSS v25 programı kullanılarak değerlendirilmiştir. Tekrarlı ölçümler arasındaki farklılıkları belirlemede parametrik olmayan Kruskal Wallis H testi (IL-6, CK), normal dağılım gösteren gruplarda one way ANOVA testi (İrisin, AMPK, GRP78,CK-MB) kullanılmış, zamanların sıra ortalamaları arasındaki fark ise anlamlılık düzeyi p<0,05 düzeyinde belirlenmiştir. Çalışma grupları incelendiğinde JA ve JB gruplarında yer alan atların E1 ve E2 müsabakalarındaki H2 dönemlerine ait değerlerin H0, H1 dönemlerindeki değerlerine göre ortalama serum irisin, IL-6, GRP78, kortizol, CK, CK-MB değerlerinde artış gözlenirken, ortalama serum AMPK, laktat, TK, TG, LDL, VLDL değerlerinde azalma tespit edilmiştir (p<0.05).Sonuç olarak, müsabakaya katılan cirit atlarında müsabakanın serum irisin, AMPK, IL-6, GRP78, kortizol, glukoz, laktat, CK, CK-MB, üre, kreatinin, AST, ALT, ALP, GGT, LDH, TK, TG, VLDL, LDL, HDL düzeyleri üzerine etkisi ortaya koyulmuştur. İrisin hormon düzeyinin ölçülmesi, egzersizin etkisi; AMPK, egzersize bağlı olarak enerji regülasyonun değerlendirilmesi, GRP78, egzersize bağlı endoplazmik retikulum stresi düzeyinin belirlenmesi, IL-6,CK, CK-MB egzersize bağlı olarak kas hasarının değerlendirilmesine katkı sağlayabileceğini düşünmekteyiz. Ancak, bu alanda daha kapsamlı ve moleküler düzeyde araştırmaların yapılmasının gerekli olduğu, egzersiz hormonu irisin, hücresel enerji sensörü AMPK, kas hasarı belirteçleri IL-6, CK parametreleri egzersiz, idman programlarının seçimi, etkinliği ve performans değerlendirilmesinde faydalı olabileceği kanaatine varılmıştır.
Javelin is one of the historical and national equestrian team sports played on horses, which is considered to be the oldest of the Turkish equestrian sports, which is accepted as the epitome of bravery, courage and masculinity played by equestrian players to keep themselves fit. It is adipokine and myokine that can be evaluated in determining the performance level of horses due to its intense presence in the muscle and called as exercise hormone. AMPK, on the other hand, is an enzyme that plays a role in energy homeostasis, which is considered as a cellular energy sensor. This study was conducted to determine the effects of competition (resting state, pre-competition and post-competition) on serum irisin, AMPK, IL-6, GRP78, cortisol hormone levels and some biochemical parameters (glukocose, laktat, CK, CK-MB, üre, kreatinin, AST, ALT, ALP, GGT, LDH, TK, TG, VLDL, LDL, HDL) horses participating in the javelin competition held in Bayburt. In this context, a total of 14 Arabian stallions aged between 4 and 10 were used in the study. Blood samples were taken from the vena jugularis from the horses included in the study during the two competition periods, in the resting state (H0), pre-competition (H1) and post-competition (H2) periods. Irisin, AMPK, IL-6, GRP78, cortisol hormone levels in blood serum samples were studied quantitatively by ELISA method, and biochemical parameters were studied quantitatively by spectrophotometric method in Cobas 8000 autoanalyzer. The serum obtained from the blood samples taken was stored at -80 °C. The statistical evaluation of the data was evaluated using the IBM SPSS v25 program. The non-parametric Kruskal Wallis H test (IL-6, CK) was used to determine the differences between repeated measurements, and the one-way ANOVA test (Irisin, AMPK, GRP78, CK-MB) was used in the groups with normal distribution. It was determined at the 0.05 level. When the study groups were examined, an increase was observed in the mean serum irisin, IL-6, GRP78, cortisol, CK, CK-MB values compared to the values of the H2 periods in the E1 and E2 competitions of the horses in the JA and JB groups compared to the values in the H0, H1 periods TC, TG, LDL, VLDL values were decreased (p<0.05). As a result, serum irisin, AMPK, IL-6, GRP78, glukoz, laktat, CK, CK-MB, üre, kreatinin, AST, ALT, ALP, GGT, LDH, TK, TG, VLDL, LDL, HDL levels have been revealed. Measurement of irisin hormone level, the effect of exercise; We think that AMPK, evaluation of exercise-induced energy regulation, GRP78, determination of exercise-induced endoplasmic reticulum stress level, IL-6,CK, CK-MB may contribute to the evaluation of exercise-induced muscle damage. However, it has been concluded that more comprehensive and molecular studies are required in this area, and that exercise hormone irisin, cellular energy sensor AMPK, muscle damage markers IL-6, CK parameters can be useful in the selection, effectiveness and performance evaluation of exercise and training programs.
Javelin is one of the historical and national equestrian team sports played on horses, which is considered to be the oldest of the Turkish equestrian sports, which is accepted as the epitome of bravery, courage and masculinity played by equestrian players to keep themselves fit. It is adipokine and myokine that can be evaluated in determining the performance level of horses due to its intense presence in the muscle and called as exercise hormone. AMPK, on the other hand, is an enzyme that plays a role in energy homeostasis, which is considered as a cellular energy sensor. This study was conducted to determine the effects of competition (resting state, pre-competition and post-competition) on serum irisin, AMPK, IL-6, GRP78, cortisol hormone levels and some biochemical parameters (glukocose, laktat, CK, CK-MB, üre, kreatinin, AST, ALT, ALP, GGT, LDH, TK, TG, VLDL, LDL, HDL) horses participating in the javelin competition held in Bayburt. In this context, a total of 14 Arabian stallions aged between 4 and 10 were used in the study. Blood samples were taken from the vena jugularis from the horses included in the study during the two competition periods, in the resting state (H0), pre-competition (H1) and post-competition (H2) periods. Irisin, AMPK, IL-6, GRP78, cortisol hormone levels in blood serum samples were studied quantitatively by ELISA method, and biochemical parameters were studied quantitatively by spectrophotometric method in Cobas 8000 autoanalyzer. The serum obtained from the blood samples taken was stored at -80 °C. The statistical evaluation of the data was evaluated using the IBM SPSS v25 program. The non-parametric Kruskal Wallis H test (IL-6, CK) was used to determine the differences between repeated measurements, and the one-way ANOVA test (Irisin, AMPK, GRP78, CK-MB) was used in the groups with normal distribution. It was determined at the 0.05 level. When the study groups were examined, an increase was observed in the mean serum irisin, IL-6, GRP78, cortisol, CK, CK-MB values compared to the values of the H2 periods in the E1 and E2 competitions of the horses in the JA and JB groups compared to the values in the H0, H1 periods TC, TG, LDL, VLDL values were decreased (p<0.05). As a result, serum irisin, AMPK, IL-6, GRP78, glukoz, laktat, CK, CK-MB, üre, kreatinin, AST, ALT, ALP, GGT, LDH, TK, TG, VLDL, LDL, HDL levels have been revealed. Measurement of irisin hormone level, the effect of exercise; We think that AMPK, evaluation of exercise-induced energy regulation, GRP78, determination of exercise-induced endoplasmic reticulum stress level, IL-6,CK, CK-MB may contribute to the evaluation of exercise-induced muscle damage. However, it has been concluded that more comprehensive and molecular studies are required in this area, and that exercise hormone irisin, cellular energy sensor AMPK, muscle damage markers IL-6, CK parameters can be useful in the selection, effectiveness and performance evaluation of exercise and training programs.
Açıklama
Lisansüstü Eğitim Enstitüsü, Organik Tarım İşletmeciliği Ana Bilim Dalı
Anahtar Kelimeler
Veteriner Hekimliği, Veterinary Medicine
