Purification of paraoxonase enzyme from the sera of patients with behcet’s disease and analyzing the effects of the drugs containing imuran (azathioprine), prednisolone (methylprednisolone) and colchium (colchicine)
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bentham Science Publishers B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, serum samples from 50 patients with the diagnosis of Behcet’s disease and 20 healthy volunteers were analyzed. The study consists of three parts. In the first part, paraoxonase (PON) activities were determined in the serum samples of 50 patients with Behcet’s disease and 20 healthy people. In the second part, equal volumes of serum samples from 50 patients were pooled and PON enzymes were purified by using Sepharose-4B-L-tyrosine tyrosine-1-naphtylamine affinity column. Optimum temperature, optimum pH, Vmax and Km values of the pure enzymes were determined. The same purification procedure was also performed in the serum samples of 20 healthy people. Electrophoretic mobility was observed (via SDS-PAGE) in the PON enzymes that were purified from the serum samples of patients with Behcet’s disease and healthy people. In the third part, in vitro effects of drugs containing azathioprine, methylprednisolone and colchicine that have already been used for the treatment of Behcet’s disease were tested on the PON enzymes of the patients with Behcet’s disease and control group. IC50 values and Ki constant values were measured and inhibition types were determined for the drugs containing azathioprine, methylprednisolone and colchicine that have already been used for the treatment of the Behcet’s disease and demonstrate in vitro inhibition effects. ©2014 Bentham Science Publishers.
Açıklama
Anahtar Kelimeler
Azathioprine, Behcet disease, Colchicine, Methylprednisolone, Paraoxonase, Purification, aryldialkylphosphatase, arylesterase, azathioprine, colchicine, methylprednisolone, sepharose, aryldialkylphosphatase, azathioprine, colchicine, enzyme inhibitor, methylprednisolone, PON1 protein, human, Article, Behcet disease, controlled study, drug blood level, drug purification, electrophoretic mobility, enzyme assay, human, in vitro study, major clinical study, maximum reaction velocity, pH, polyacrylamide gel electrophoresis, priority journal, protein determination, temperature, antagonists and inhibitors, Behcet Syndrome, blood, case control study, enzymology, kinetics, Aryldialkylphosphatase, Azathioprine, Behcet Syndrome, Case-Control Studies, Colchicine, Enzyme Inhibitors, Humans, Kinetics, Methylprednisolone, Azathioprine, Behcet disease, Colchicine, Methylprednisolone, Paraoxonase, Purification, aryldialkylphosphatase, arylesterase, azathioprine, colchicine, methylprednisolone, sepharose, aryldialkylphosphatase, azathioprine, colchicine, enzyme inhibitor, methylprednisolone, PON1 protein, human, Article, Behcet disease, controlled study, drug blood level, drug purification, electrophoretic mobility, enzyme assay, human, in vitro study, major clinical study, maximum reaction velocity, pH, polyacrylamide gel electrophoresis, priority journal, protein determination, temperature, antagonists and inhibitors, Behcet Syndrome, blood, case control study, enzymology, kinetics, Aryldialkylphosphatase, Azathioprine, Behcet Syndrome, Case-Control Studies, Colchicine, Enzyme Inhibitors, Humans, Kinetics, Methylprednisolone
Kaynak
Drug Metabolism Letters
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
8
Sayı
1
