Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

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dc.contributor.authorErmiş, Ertan
dc.contributor.authorPoyraz, Ecem
dc.contributor.authorDertli, Enes
dc.contributor.authorYilmaz, Mustafa Tahsin
dc.date.accessioned2024-10-04T19:06:34Z
dc.date.available2024-10-04T19:06:34Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractResponse surface methodology was used to determine the exopolysaccharide production of Lactobacillus brevis E25. The effects of three variables, temperature (30, 36 and 42°C), incubation time (18, 33 and 48 h) and initial pH of growth medium (4.5, 5.5, and 6.5) were studied. Under optimum growth conditions, the amount of exopolysaccharide derived from Lactobacillus brevis E25 ranged from 10 to 35 gL-1. The size of EPS molecules ranged from 105 to 107Da. Infra red spectrum analysis showed characteristics absorption peaks indicating the presence of -OH, C=O and C-H groups. Furthermore, only glucose was detected as monosaccharide in exopolysaccharide structure, revealing that the structure of exopolysaccharide is a homopolymeric glucan type. Based on the differential scanning calorimeter thermograms, exopolysaccharide’s melting temperature was observed around 116 °C.Keywords: Lactic acid bacteria, exopolysaccharide, response surface methodology, molecular characterizationen_US
dc.identifier.doi10.16984/saufenbilder.545929
dc.identifier.endpage160en_US
dc.identifier.issn1301-4048
dc.identifier.issn2147-835X
dc.identifier.issue1en_US
dc.identifier.startpage151en_US
dc.identifier.trdizinid472272en_US
dc.identifier.urihttps://doi.org/10.16984/saufenbilder.545929
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/472272
dc.identifier.urihttp://hdl.handle.net/20.500.12403/4588
dc.identifier.volume24en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofSakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleOptimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterizationen_US
dc.typeArticleen_US

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