Purification and properties of carbonic anhydrase from bone marrow

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dc.authorid8064145700
dc.authorid6506121388
dc.authorid7006472528
dc.authorid35511537500
dc.contributor.authorTasgin E.
dc.contributor.authorNadaroglu H.
dc.contributor.authorDemir Y.
dc.contributor.authorDemir N.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:44:57Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:44:57Z
dc.date.issued2009
dc.departmentBayburt Üniversitesien_US
dc.description.abstractIn this work, the carbonic anhydrase was purified from bovine bone marrow and investigated its kinetic properties. Carbonic anhydrase was purified from bovine bone marrow using affinity chromatography by sepharose 4B-L-tyrosine sulphanilamide. During purification steps, the activity of enzyme was measured using p-nitrophenyl acetate at pH: 7.4. Optimum pH and optimum temperature values for bovine bone marrow carbonic anhydrase were determined and then K m and Vmax values for the same substrate were obtained by means of Linewearver-Burk graphics. The purification degree for bovine bone marrow was calculated. The Vmax, and Km values at optimum pH and at 20 °C for the substrate (p-nitrophenyl acetate) were 120.418 u?mol/L min and 2.409 x 103 mM, respectively. The K1 and I50 values for sulfanilamide, KSCN, NaN3 and acetazolamide were determined in bovine bone marrow carbonic anhydrase.en_US
dc.identifier.endpage5122
dc.identifier.issn0970-7077
dc.identifier.issue7
dc.identifier.startpage5117
dc.identifier.urihttps://hdl.handle.net/20.500.12403/978
dc.identifier.volume21
dc.language.isoenen_US
dc.relation.ispartofAsian Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBone marrow
dc.subjectCarbonic anhydrase
dc.subjectKinetics
dc.subjectBone marrow
dc.subjectCarbonic anhydrase
dc.subjectKinetics
dc.titlePurification and properties of carbonic anhydrase from bone marrowen_US
dc.typeArticleen_US

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