The Phenolic Profile and Anti-Inflammatory Effect of Ethanolic Extract of Polish Propolis on Activated Human Gingival Fibroblasts-1 Cell Line

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dc.authoridKlosek, Malgorzata/0000-0002-9266-2292
dc.authoridBalwierz, Radoslaw/0000-0002-6173-2702
dc.authoridOlczyk, Pawel/0000-0001-7387-9587
dc.authoridCzuba, Zenon/0000-0001-8216-4495
dc.authoridKurek-Gorecka, Anna/0000-0002-2006-6715
dc.contributor.authorKurek-Gorecka, Anna
dc.contributor.authorKlosek, Malgorzata
dc.contributor.authorPietsz, Grazyna
dc.contributor.authorCzuba, Zenon P.
dc.contributor.authorKolayli, Sevgi
dc.contributor.authorCan, Zehra
dc.contributor.authorBalwierz, Radoslaw
dc.date.accessioned2024-10-04T18:53:58Z
dc.date.available2024-10-04T18:53:58Z
dc.date.issued2023
dc.departmentBayburt Üniversitesien_US
dc.description.abstractPropolis, owing to its antibacterial and anti-inflammatory properties, acts as a cariostatic agent, capable of preventing the accumulation of dental plaque and inhibiting inflammation. The anti-inflammatory properties of propolis are attributed to caffeic acid phenethyl ester (CAPE), which is present in European propolis. The objective of the conducted study was to assess the anti-inflammatory effects of the Polish ethanolic extract of propolis (EEP) and isolated CAPE on stimulated with LPS and IFN-alpha, as well as the combination of LPS and IFN-alpha. The cytotoxicity of the tested compounds was determined using the MTT assay. The concentrations of specific cytokines released by the HGF-1 cell line following treatment with EEP (25-50 mu g/mL) or CAPE (25-50 mu g/mL) were assessed in the culture supernatant. In the tested concentrations, both CAPE and EEP did not exert cytotoxic effects. Our results demonstrate that CAPE reduces TNF-alpha and IL-6 in contrast to EEP. Propolis seems effective in stimulating HGF-1 to release IL-6 and IL-8. A statistically significant difference was observed for IL-8 in HGF-1 stimulated by LPS+IFN-alpha and treated EEP at a concentration of 50 mu g/mL (p = 0.021201). Moreover, we observed that CAPE demonstrates a stronger interaction with IL-8 compared to EEP, especially when CAPE was administered at a concentration of 50 mu g/mL after LPS + IFN-alpha stimulation (p = 0.0005). Analysis of the phenolic profile performed by high-performance liquid chromatography allowed identification and quantification in the EEP sample of six phenolic acids, five flavonoids, and one aromatic ester-CAPE. Propolis and its compound-CAPE-exhibit immunomodulatory properties that influence the inflammatory process. Further studies may contribute to explaining the immunomodulatory action of EEP and CAPE and bring comprehensive conclusions.en_US
dc.description.sponsorshipMedical University of Silesiaen_US
dc.description.sponsorshipThis work is the product of an Erasmus + mobility project.en_US
dc.identifier.doi10.3390/molecules28227477
dc.identifier.issn1420-3049
dc.identifier.issue22en_US
dc.identifier.pmid38005199en_US
dc.identifier.urihttps://doi.org/10.3390/molecules28227477
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3811
dc.identifier.volume28en_US
dc.identifier.wosWOS:001113800700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofMoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcaffeic acid phenethyl esteren_US
dc.subjectcytokinesen_US
dc.subjectinflammationen_US
dc.subjectin vitro modelen_US
dc.subjectpolyphenolsen_US
dc.subjectpropolisen_US
dc.titleThe Phenolic Profile and Anti-Inflammatory Effect of Ethanolic Extract of Polish Propolis on Activated Human Gingival Fibroblasts-1 Cell Lineen_US
dc.typeArticleen_US

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