Synthesis and in Vitro Toxicity Assessment of Different Nano-Calcium Phosphate Nanoparticles

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dc.authoridBakan Misirlioglu, Feray/0000-0001-6467-8943
dc.authoridMardinoglu, Adil/0000-0002-4254-6090
dc.authoridARSLAN, Mehmet Enes/0000-0002-1600-2305
dc.authoridcacciatore, ivana/0000-0001-6253-0443
dc.contributor.authorTogar, Basak
dc.contributor.authorTurkez, Hasan
dc.contributor.authorBakan, Feray
dc.contributor.authorArslan, Mehmet Enes
dc.contributor.authorTatar, Abdulgani
dc.contributor.authorCacciatore, Ivana
dc.contributor.authorHacimuftuoglu, Ahmet
dc.date.accessioned2024-10-04T18:53:49Z
dc.date.available2024-10-04T18:53:49Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractNanoscale biomaterials are commonly used in a wide range of biomedical applications such as bone graft substitutes, gene delivery systems, and biologically active agents. On the other hand, the cytotoxic potential of these particles hasn't yet been studied comprehensively to understand whether or not they exert any negative impact on the cellular structures. Here, we undertook the synthesis of beta-tricalcium phosphate (beta-TCP) and biphasic tricalcium phosphate (BCP) nanoparticles (NPs) and determine their concentration-dependent toxic effects in human fetal osteoblastic (hFOB 1.19) cell line. Firstly, BCP and beta-TCP were synthesized using a water-based precipitation technique and characterized by X-Ray Diffraction (XRD), Raman Spectroscopy, and Transmission Electron Microscopy (TEM). The cytological effects of beta-TCP and BCP at different concentrations (0-640 ppm) were evaluated by using 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The total oxidative status (TOS) parameter was used for investigating oxidative stress potentials of the NPs. In addition, the study assessed the DNA damage product 8-hydroxy-2'-deoxyguanosine (8-Oxo-dG) level in hFOB 1.19 cell cultures. The results indicated that the beta-TCP (above 320 ppm) and BCP (above 80 ppm) NPs exhibited cytotoxicity effects on high concentrations. It was also observed that the oxidative stress increased relatively as the concentrations of NPs increased, aligning with the cytotoxicity results. However, the NPs concentrations of 160 ppm and above increased the level of 8-OH-dG. Consequently, there is a need for more systematic in vivo and in vitro approaches to the toxic effects of both nanoparticles.en_US
dc.identifier.doi10.1590/1678-4324-2022200784
dc.identifier.issn1516-8913
dc.identifier.issn1678-4324
dc.identifier.scopus2-s2.0-85130055817en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1590/1678-4324-2022200784
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3720
dc.identifier.volume65en_US
dc.identifier.wosWOS:000768802200001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInst Tecnologia Paranaen_US
dc.relation.ispartofBrazilian Archives of Biology and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbeta-Tricalcium phosphateen_US
dc.subjectbiphasic calcium phosphateen_US
dc.subjectnanoparticleen_US
dc.subjectcytotoxicityen_US
dc.subjecttotal oxidative statusen_US
dc.subjectgenotoxicityen_US
dc.titleSynthesis and in Vitro Toxicity Assessment of Different Nano-Calcium Phosphate Nanoparticlesen_US
dc.typeArticleen_US

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