Ferulago pauciradiata Boiss. & Heldr. Effects of Prantschimgin Compound Isolated from the Root of the Plant on LPS-Induced Inflammation Model in the L929 Cell Line
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Tarih
2021
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info:eu-repo/semantics/openAccess
Özet
Investigation of the effects of prantchimgin (Prn) compound isolated from the root part of the Ferulago pauciradiata (Apiaceae) plant in the lipopolysaccharide (LPS) cytotoxicity model created in the L929 cell line. L929 cell lines were applied in a 5% CO2 incubator at 37 °C, using standard cell culture procedures, LPS was applied with Prn pure substance with a final concentration of 2 µL and 1 µL after 1 hour. Following the LPS application, the MTT protocol, a colorimetric method, was applied to determine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times. IC50 values were calculated as 0.28 µg/mL for Pnr and 1 µg/mL for LPS. Application of LPS to L929 cell lines caused a significant decrease in cellular index depending on time. Also, in the Prn + LPS groups, it was found that the decreased cell index significantly increased even closer to the control compared to the LPS applied group. It was found that cyctoxicity and cell damage caused by LPS applied to the L929 cell line improved after Pnr application.
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Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi
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Cilt
14
Sayı
2
