Optimization of Asymmetric Bioreduction Conditions of 2-methyl-1-phenylpropan-1-one by Lactobacillus fermentum BY35 Using I-Optimal Design-Based Model

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dc.contributor.authorAksuoglu, Selmani
dc.contributor.authorOzdemir, Akin
dc.contributor.authorSerencam, Huseyin
dc.contributor.authorDertli, Enes
dc.contributor.authorSahin, Engin
dc.date.accessioned2024-10-04T18:52:33Z
dc.date.available2024-10-04T18:52:33Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractThe bioreduction of prochiral ketones offers efficient access to chiral secondary alcohols, which are potentially beneficial precursors for producing many biologically active compounds and natural products. This bioreduction process can be affected by different parameters when whole-cell of biocatalysts such as Lactic Acid Bacteria strains are applied. In this context, this paper proposed an I-optimal design-based model to optimize culture parameters such as temperature, pH, incubation period, and agitation speed for asymmetric bioreduction of 2-methyl-1-phenylpropan-1-one (1) with Lactobacillus fermentum BY35 as a biocatalyst while achieving the highest conversion rate (cr) and enantiomeric excess (ee). The optimum settings of the four culture parameters and the cr and ee values were found using the proposed optimization model as follows: pH = 6.5, temperature = 25 degrees C, incubation period = 38.5 h, agitation speed = 200 rpm, the ee value = 98.78%, and the cr value = 98.92%. After the validation of the process, the cr and ee values were found to be > 99% and > 99%, respectively, while using the optimum operating conditions from the optimization model. Thus, the results of the optimization model are consistent with the results of the validation experiment. It is also noted that this paper is the first to optimize culture parameters using the proposed I-optimal design-based model for an asymmetric reduction.en_US
dc.identifier.doi10.1007/s13369-021-06434-5
dc.identifier.endpage6514en_US
dc.identifier.issn2193-567X
dc.identifier.issn2191-4281
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85123487727en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage6505en_US
dc.identifier.urihttps://doi.org/10.1007/s13369-021-06434-5
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3545
dc.identifier.volume47en_US
dc.identifier.wosWOS:000745604700004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofArabian Journal For Science and Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectI-optimal designen_US
dc.subjectOptimizationen_US
dc.subjectChiral secondary alcoholsen_US
dc.subjectWhole-cell biocatalysten_US
dc.subjectAsymmetric bioreductionen_US
dc.subjectBiotransformationsen_US
dc.titleOptimization of Asymmetric Bioreduction Conditions of 2-methyl-1-phenylpropan-1-one by Lactobacillus fermentum BY35 Using I-Optimal Design-Based Modelen_US
dc.typeArticleen_US

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