Investigation of alterations in PIK3CA and OCT-4 gene expression in breast cancer

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dc.authoridilvan, Sennur/0000-0002-6746-6599
dc.authoridDirican, Ebubekir/0000-0001-9260-5223
dc.contributor.authorDirican, Ebubekir
dc.contributor.authorKankaya, Burak
dc.contributor.authorBuyukasik, Suleyman
dc.contributor.authorAlis, Halil
dc.contributor.authorVelidedeoglu, Mehmet
dc.contributor.authorIlvan, Sennur
dc.contributor.authorIlvan, Ahmet
dc.date.accessioned2024-10-04T18:51:10Z
dc.date.available2024-10-04T18:51:10Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractBreast cancer (BCa) is one of the most prevalent cancer amongst women and PIK3CA mutations have a crucial role in carcinogenesis. On the other hand, Octamer-Binding Transcription Factor 4 (OCT-4) is common biomarkers of cancer stem cells, which provide to the pathological processes of numerous carcinomas. In this study, we evaluated the association of OCT-4 gene expression levels with the PIK3CA mutation in BCa patients. The mRNA expression level of the OCT-4 gene was detected by qPCR in 44 BCa patients and 30 BCa patients control have positive PIK3CA mutations. Exon 9 and exon 20 of PIK3CA mutations were identified by sequencing. OCT-4 gene expression and PIK3CA mutations were also clinically evaluated in BCa patients. The frequency of PIK3CA mutation in 44 new cases were 32%. PIK3CA mutations were significantly associated with HER2-positive (p = 0.0271) status, Ki-67 status (p = 0.0434) and patient age (p = 0.0149). OCT-4 mRNA expression was significantly higher in the BCa tissue in comparison to normal breast tissue (p = 0.0305). A significant correlation was found between expression of OCT-4 and node status (p = 0.0190). OCT-4 mRNA expression was higher in the PIK3CA mutation tissue than the wild type tissue. However, no association was found between the expression of OCT-4 and the PIK3CA mutation or wild type samples (p = 0.7908). Our results provided that OCT-4 gene and PIK3CA mutations may have a role in development of BCa. OCT-4 mRNA expression level and PIK3CA mutations have a potential important for BCa diagnosis or treatment.en_US
dc.description.sponsorshipResearch Foundation of Istanbul Aydin University (BAP) [2019/1-1648]en_US
dc.description.sponsorshipThis work was supported by grants (2019/1-1648 to E.D.) from the Research Foundation of Istanbul Aydin University (BAP).en_US
dc.identifier.doi10.1016/j.genrep.2020.100942
dc.identifier.issn2452-0144
dc.identifier.scopus2-s2.0-85094956501en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.1016/j.genrep.2020.100942
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3412
dc.identifier.volume21en_US
dc.identifier.wosWOS:000598178300012en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofGene Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOCT-4en_US
dc.subjectBreast canceren_US
dc.subjectPIK3CAen_US
dc.subjectqPCRen_US
dc.subjectBiomarkeren_US
dc.titleInvestigation of alterations in PIK3CA and OCT-4 gene expression in breast canceren_US
dc.typeArticleen_US

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