Suberosin Alleviates Sepsis-Induced Lung Injury in A Rat Model of Cecal Ligation and Puncture

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dc.authoridBOLAT, ISMAIL/0000-0003-1398-7046
dc.authoridKilic, Ceyda Sibel/0000-0003-2905-7628
dc.contributor.authorUzuncakmak, Sevgi Karabulut
dc.contributor.authorHalici, Zekai
dc.contributor.authorKarakaya, Songul
dc.contributor.authorKutlu, Zerrin
dc.contributor.authorSaglam, Yavuz Selim
dc.contributor.authorBolat, Ismail
dc.contributor.authorAydin, Pelin
dc.date.accessioned2024-10-04T18:53:48Z
dc.date.available2024-10-04T18:53:48Z
dc.date.issued2023
dc.departmentBayburt Üniversitesien_US
dc.description.abstractBackground/aims Sepsis is one of the major problems encountered in intensive care units, causing organ damage and increasing mortality. Suberosin (SBR) is a type of coumarin with antioxidant and anti-inflammatory activities. The goal of this study is to explore the protective effects of SBR on the lungs in a rat model of sepsis. Methods Male Wistar rats were utilized in this study. A cecal ligation and puncture (CLP) model was applied to induce sepsis. Rats were separated into six groups with nine animals in each group, including healthy control, SBR, CLP, and CLP + SBR (5, 10, and 20 mg/kg) groups. Superoxide dismutase (SOD), glutathione (GSH) enzyme activities, and malondialdehyde (MDA) level were measured via enzyme-linked immunosorbent assay (ELISA). The messenger RNA (mRNA) expressions of tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) were evaluated by real-time polymerase chain reaction (RT-PCR). Histopathological changes in the lungs were investigated with hematoxylin and eosin (H&E). Results MDA levels and GSH and SOD enzyme activities were negatively affected in the CLP group, but SBR treatment ameliorated these oxidative stress parameters in the SBR1-3 groups (p< 0.05). The mRNA expressions of TNF-alpha and IL-1 beta were increased in the CLP group, and SBR treatment decreased those expression levels in a dose-dependent manner (p < 0.05). Organ damage and necrosis were seen in the CLP group and were alleviated in the SBR3 group. Immunohistochemical (IHC) analysis of lung tissues demonstrated decreased TNF-alpha and IL-1 beta immunopositivity in the SBR1-3 groups (p< 0.05). Conclusions SBR ameliorated sepsis-related lung injury in a dose-dependent manner. This compound has significant potential as a future agent in the treatment of sepsis.en_US
dc.identifier.doi10.1080/08941939.2022.2136802
dc.identifier.endpage9en_US
dc.identifier.issn0894-1939
dc.identifier.issn1521-0553
dc.identifier.issue1en_US
dc.identifier.pmid36345760en_US
dc.identifier.scopus2-s2.0-85141894711en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1080/08941939.2022.2136802
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3711
dc.identifier.volume36en_US
dc.identifier.wosWOS:000879947700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofJournal of Investigative Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectoxidative stressen_US
dc.subjectraten_US
dc.subjectsepsisen_US
dc.subjectsuberosinen_US
dc.subjectlung injuryen_US
dc.titleSuberosin Alleviates Sepsis-Induced Lung Injury in A Rat Model of Cecal Ligation and Punctureen_US
dc.typeArticleen_US

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