Human serum paraoxonase-1 (hPON1): In vitro inhibition effects of moxifloxacin hydrochloride, levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodium

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Tarih

2015

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Taylor and Francis Ltd

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

In this study, we investigated the effects of antibacterial drugs (moxifloxacin hydrochloride, levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodium) on human serum paraoxonase-1 (hPON1) enzyme activity from human serum in vitro conditions. For this purpose, hPON1 enzyme was purified from human serum using simple chromatographic methods. The antibacterial drugs exhibited inhibitory effects on hPON1 at low concentrations. K<inf>i</inf> constants were calculated to be 2.641 ± 0.040 mM, 5.525 ± 0.817 mM, 35.092 ± 1.093 mM, 252.762 ± 5.749 mM and 499.244 ± 10.149 mM, respectively. The inhibition mechanism of moxifloxacin hydrochloride was competitive, whereas levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodium were noncompetitive inhibitors. © 2014 Informa UK Ltd. All rights reserved.

Açıklama

Anahtar Kelimeler

Cefepime hydrochloride, cefotaxime sodium, ceftizoxime sodium, inhibition, levofloxacin hemihidrate, moxifloxacin hydrochloride, paraoxonase, aryldialkylphosphatase 1, cefepime, cefotaxime, ceftizoxime, levofloxacin, moxifloxacin, aryldialkylphosphatase, cefepime, cefotaxime, ceftizoxime, cephalosporin derivative, levofloxacin, moxifloxacin, PON1 protein, human, quinolone derivative, anion exchange chromatography, Article, enzyme activity, enzyme inhibition, gel filtration chromatography, in vitro study, precipitation, priority journal, serum, antagonists and inhibitors, blood, human, Aryldialkylphosphatase, Cefotaxime, Ceftizoxime, Cephalosporins, Fluoroquinolones, Humans, In Vitro Techniques, Levofloxacin, Cefepime hydrochloride, cefotaxime sodium, ceftizoxime sodium, inhibition, levofloxacin hemihidrate, moxifloxacin hydrochloride, paraoxonase, aryldialkylphosphatase 1, cefepime, cefotaxime, ceftizoxime, levofloxacin, moxifloxacin, aryldialkylphosphatase, cefepime, cefotaxime, ceftizoxime, cephalosporin derivative, levofloxacin, moxifloxacin, PON1 protein, human, quinolone derivative, anion exchange chromatography, Article, enzyme activity, enzyme inhibition, gel filtration chromatography, in vitro study, precipitation, priority journal, serum, antagonists and inhibitors, blood, human, Aryldialkylphosphatase, Cefotaxime, Ceftizoxime, Cephalosporins, Fluoroquinolones, Humans, In Vitro Techniques, Levofloxacin

Kaynak

Journal of Enzyme Inhibition and Medicinal Chemistry

WoS Q Değeri

Q1

Scopus Q Değeri

Q1

Cilt

30

Sayı

4

Künye