Human serum paraoxonase-1 (hPON1): In vitro inhibition effects of moxifloxacin hydrochloride, levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodium
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, we investigated the effects of antibacterial drugs (moxifloxacin hydrochloride, levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodium) on human serum paraoxonase-1 (hPON1) enzyme activity from human serum in vitro conditions. For this purpose, hPON1 enzyme was purified from human serum using simple chromatographic methods. The antibacterial drugs exhibited inhibitory effects on hPON1 at low concentrations. K<inf>i</inf> constants were calculated to be 2.641 ± 0.040 mM, 5.525 ± 0.817 mM, 35.092 ± 1.093 mM, 252.762 ± 5.749 mM and 499.244 ± 10.149 mM, respectively. The inhibition mechanism of moxifloxacin hydrochloride was competitive, whereas levofloxacin hemihidrate, cefepime hydrochloride, cefotaxime sodium and ceftizoxime sodium were noncompetitive inhibitors. © 2014 Informa UK Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
Cefepime hydrochloride, cefotaxime sodium, ceftizoxime sodium, inhibition, levofloxacin hemihidrate, moxifloxacin hydrochloride, paraoxonase, aryldialkylphosphatase 1, cefepime, cefotaxime, ceftizoxime, levofloxacin, moxifloxacin, aryldialkylphosphatase, cefepime, cefotaxime, ceftizoxime, cephalosporin derivative, levofloxacin, moxifloxacin, PON1 protein, human, quinolone derivative, anion exchange chromatography, Article, enzyme activity, enzyme inhibition, gel filtration chromatography, in vitro study, precipitation, priority journal, serum, antagonists and inhibitors, blood, human, Aryldialkylphosphatase, Cefotaxime, Ceftizoxime, Cephalosporins, Fluoroquinolones, Humans, In Vitro Techniques, Levofloxacin, Cefepime hydrochloride, cefotaxime sodium, ceftizoxime sodium, inhibition, levofloxacin hemihidrate, moxifloxacin hydrochloride, paraoxonase, aryldialkylphosphatase 1, cefepime, cefotaxime, ceftizoxime, levofloxacin, moxifloxacin, aryldialkylphosphatase, cefepime, cefotaxime, ceftizoxime, cephalosporin derivative, levofloxacin, moxifloxacin, PON1 protein, human, quinolone derivative, anion exchange chromatography, Article, enzyme activity, enzyme inhibition, gel filtration chromatography, in vitro study, precipitation, priority journal, serum, antagonists and inhibitors, blood, human, Aryldialkylphosphatase, Cefotaxime, Ceftizoxime, Cephalosporins, Fluoroquinolones, Humans, In Vitro Techniques, Levofloxacin
Kaynak
Journal of Enzyme Inhibition and Medicinal Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
30
Sayı
4
