Characterization of a glucansucrase from Lactobacillus reuteri E81 and production of malto-oligosaccharides

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dc.authoridYuzer, Mustafa Onur/0000-0002-8192-2298
dc.authoridISPIRLI, HUMEYRA/0000-0002-7601-0374
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorYuzer, Mustafa Onur
dc.contributor.authorSkory, Christopher
dc.contributor.authorColquhoun, Ian J.
dc.contributor.authorSagdic, Osman
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:48:31Z
dc.date.available2024-10-04T18:48:31Z
dc.date.issued2019
dc.departmentBayburt Üniversitesien_US
dc.description.abstractGlucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of alpha-glucans with different structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified and heterologously expressed. Alignments of GTFA with other glucansucrases revealed its novelty and a putative 3D model structure was obtained. The biochemical properties of the truncated enzyme without the N-terminal variable region, GTFA-Delta N, was characterized. The K-m and V-max were found to be 7.5 mM and 1.49 IU/mg, respectively, and it showed optimum activities at pH 7 and at 50 degrees C. The GTFA-Delta N produced in vitro an alpha-glucan with (alpha 1 -> 3) and (alpha 1 -> 6) glycosidic linkages using sucrose as the substrate. Importantly, GTFA-Delta N synthesized DP = 9 oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively, as detected by TLC, HPLC, LC-MS and NMR analysis.en_US
dc.description.sponsorshipTUBITAK [116O523]en_US
dc.description.sponsorshipThis study was financially supported by TUBITAK with the Grant number 116O523.en_US
dc.identifier.doi10.1080/10242422.2019.1593969
dc.identifier.endpage430en_US
dc.identifier.issn1024-2422
dc.identifier.issn1029-2446
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85065527836en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage421en_US
dc.identifier.urihttps://doi.org/10.1080/10242422.2019.1593969
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3076
dc.identifier.volume37en_US
dc.identifier.wosWOS:000469706400001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiocatalysis and Biotransformationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlucansucraseen_US
dc.subjectglucanen_US
dc.subjectmalto-oligosaccharidesen_US
dc.titleCharacterization of a glucansucrase from Lactobacillus reuteri E81 and production of malto-oligosaccharidesen_US
dc.typeArticleen_US

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