Synthesis and characterization of cellobiose-derived oligosaccharides with Bifidogenic activity by glucansucrase E81

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dc.contributor.authorIspirli, Humeyra
dc.contributor.authorBowman, Michael J.
dc.contributor.authorSkory, Christopher D.
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:53:49Z
dc.date.available2024-10-04T18:53:49Z
dc.date.issued2021
dc.departmentBayburt Üniversitesien_US
dc.description.abstractCellobiose-derived oligosaccharides were produced from acceptor reactions with glucansucrase E81 and their prebiotic potentials were determined in this study. The major product was a mixture of trisaccharides formed by the addition of an alpha-linked glucose to cellobiose at either the 2-O position of the reducing end (86%) glucose or the 6-O position of the non-reducing end glucose (11%) demonstrated by NMR spectroscopy and LC-MS-LTQ analysis. The purified DP 3 cellobiose-derived oligosaccharides were then tested for in vitro human gastric juice and alpha-amylase digestion and no digestion was observed during the 6 h digestion period. Importantly, DP 3 cellobiose-derived oligosaccharides promoted the growth of Bifidobacteria at high levels demonstrating a Bifidogenic effect and a mild level of proliferation was observed for Lactobacilli suggesting the prebiotic roles of cellobiose-derived oligosaccharides.en_US
dc.description.sponsorshipTUBITAK [116O523]; U.S. Department of Agriculture, Agricultural Research Serviceen_US
dc.description.sponsorshipThe authors are thankful to TUBITAK for the financial support for this study with the grant number 116O523. This work was supported in part by the U.S. Department of Agriculture, Agricultural Research Service.en_US
dc.identifier.doi10.1016/j.fbio.2021.101388
dc.identifier.issn2212-4292
dc.identifier.issn2212-4306
dc.identifier.scopus2-s2.0-85116322339en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.fbio.2021.101388
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3719
dc.identifier.volume44en_US
dc.identifier.wosWOS:000710483900002en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofFood Bioscienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGlucansucraseen_US
dc.subjectPrebiotic oligosaccharidesen_US
dc.subjectCellobiose-derived oligosaccharidesen_US
dc.titleSynthesis and characterization of cellobiose-derived oligosaccharides with Bifidogenic activity by glucansucrase E81en_US
dc.typeArticleen_US

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