Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions

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dc.authoridISPIRLI, HUMEYRA/0000-0002-7601-0374
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorColquhoun, Ian J.
dc.contributor.authorSahin, Engin
dc.contributor.authorSagdic, Osman
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:52:41Z
dc.date.available2024-10-04T18:52:41Z
dc.date.issued2019
dc.departmentBayburt Üniversitesien_US
dc.description.abstractGentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively. The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis. The glycosylation of the gentiobiose with alpha-(1 -> 6) linkages and alpha-(1 -> 3) linkages was shown by H-1 and C-13 NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides. The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains. Gentiobiosederived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-alpha cytokines in HT29 cells in a dose dependent manner. This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.en_US
dc.description.sponsorshipTUBITAK [116O523]en_US
dc.description.sponsorshipThis study was financially supported by TUBITAK with the grant number 116O523.en_US
dc.identifier.doi10.1016/j.carres.2019.107837
dc.identifier.issn0008-6215
dc.identifier.issn1873-426X
dc.identifier.pmid31655418en_US
dc.identifier.scopus2-s2.0-85073676607en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.carres.2019.107837
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3616
dc.identifier.volume486en_US
dc.identifier.wosWOS:000496174300018en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofCarbohydrate Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlucansucraseen_US
dc.subjectGentiobiose-derived oligosaccharidesen_US
dc.subjectImmune modulationen_US
dc.subjectPrebioticen_US
dc.titlePreparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functionsen_US
dc.typeArticleen_US

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