Determination of anti-cancer effects of Nigella sativa seed oil on MCF7 breast and AGS gastric cancer cells

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dc.authoridCINAR, IRFAN/0000-0002-9826-2556
dc.authoridGIDIK, Betul/0000-0002-3617-899X
dc.contributor.authorCinar, Irfan
dc.contributor.authorGidik, Betul
dc.contributor.authorDirican, Ebubekir
dc.date.accessioned2024-10-04T18:49:26Z
dc.date.available2024-10-04T18:49:26Z
dc.date.issued2024
dc.departmentBayburt Üniversitesien_US
dc.description.abstractBackground This study aimed to investigate the cytotoxic, apoptotic, invasion, metastasis, and heat shock proteins (HSPs) effects of N. sativa oil on breast and gastric cancer cells. Methods We assessed the cytotoxic and apoptotic effects of various concentrations of N. sativa oil (10-50-100-200 mu g/mL) on MCF7 breast cancer and AGS, an adenocarcinoma of the gastric cell line, at 24, 48 and 72 h using the MTT test. Additionally, the expression of the Caspase-3, BCL2/Bax, MMP2-9 and HSP60-70 gene was examined using RT-PCR in cell lines treating with N. sativa. Results The MTT experiments demonstrate that N. sativa has a time and dose-dependent inhibitory effect on the proliferation of MCF7 and AGS cancer cells. The vitality rates of MCF7 and AGS cells treated with N. sativa were 77.04-67.50% at 24 h, 65.28-39.14% at 48 h, and 48.95-32.31% at 72 h. The doses of 100 and 200 mu g/mL were shown to be the most effective on both cancer cells. RT-PCR analysis revealed that N. sativa oil extract increased caspase-3 levels in both cell lines at higher concentrations and suppressed BCL2/Bax levels. Exposure of MCF7 and AGS cell lines to N. sativa caused a significant decrease in the expression of MMP2-9 and HSP60-70 genes over time, particularly at a dosage of 200 mu g/mL compared to the control group (p < 0.05). Conclusions Our findings indicate that N. sativa oil has a dose-dependent effect on cytotoxicity and the expression of apoptotic, heat shock proteins, and matrix metalloproteinases genes in breast and gastric cancer.en_US
dc.identifier.doi10.1007/s11033-024-09453-1
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1en_US
dc.identifier.pmid38578469en_US
dc.identifier.scopus2-s2.0-85189778144en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-024-09453-1
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3148
dc.identifier.volume51en_US
dc.identifier.wosWOS:001197733900015en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectN. sativaen_US
dc.subjectMCF7en_US
dc.subjectAGSen_US
dc.subjectHSPsen_US
dc.subjectMMPsen_US
dc.subjectRT PCRen_US
dc.titleDetermination of anti-cancer effects of Nigella sativa seed oil on MCF7 breast and AGS gastric cancer cellsen_US
dc.typeArticleen_US

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