Optimization of asymmetric bioreduction conditions of 1-(thiophen-2-yl)ethanone by Weissella cibaria N9 using a desirability function-embedded face-centered optimization model

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dc.contributor.authorBolubaid, Mohammed
dc.contributor.authorOzdemir, Akin
dc.contributor.authorDertli, Enes
dc.contributor.authorAlamoudi, Mohammed
dc.contributor.authorTaylan, Osman
dc.contributor.authorKaraboga, Dervis
dc.contributor.authorYilmaz, Mustafa Tahsin
dc.date.accessioned2024-10-04T18:52:33Z
dc.date.available2024-10-04T18:52:33Z
dc.date.issued2023
dc.departmentBayburt Üniversitesien_US
dc.description.abstractProchiral ketones can be effectively bio-reduced to chiral secondary alcohols by whole-cell biocatalysts, which are possible useful precursors to synthesize physiologically active chemicals and natural products. When whole-cell biocatalysts strains are used, bioreduction process can be influenced by various cultural factors, and it is vital to optimize these factors that affect selectivity, conversion rate, and yield. In this study, Weissella cibaria N9 was used as whole-cell biocatalyst for bioreduction of 1-(thiophen-2-yl)ethanone, and cultural design factors were optimized using a desirability function-embedded face-centered optimization model. For this, effects of pH (4.5-5.5-6.5, x(1)), (2) temperature (25-30-35 degrees C, x(2)), (3) incubation period (24-48-72 h, x(3)), and (4) agitation speed (100-150-200 rpm, x(4)) on two response variables; (1) ee (%) and (2) cr (%) were tested. Next, desirability function-embedded face-centered optimization model revealed that a pH of 6.43, a temperature of 26.04 degrees C, an incubation period of 52.41 h, and an agitation speed of 150 rpm were the optimum levels and the estimated ee and cr responses were 99.31% and 98.16%, respectively. Importantly, the actual experimental ee and cr responses were similar to the estimated values indicating the capability of the offered desirability function-embedded face-centered optimization model when using the optimum cultural conditions.en_US
dc.description.sponsorshipDeanship of Scientific Research (DSR), King Abdulaziz University, Jeddah, Saudi Arabia [RG-27-135-42]en_US
dc.description.sponsorshipThis research work was funded by the Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah, Saudi Arabia under grant No. [RG-27-135-42]. Thus, the authors gratefully acknowledge the technical and financial support from the DSR. Also, we would like to thank Bayburt University Central Research Laboratory for the HPLC analysis.en_US
dc.identifier.doi10.1080/10826068.2023.2185898
dc.identifier.endpage1262en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue10en_US
dc.identifier.pmid36876855en_US
dc.identifier.scopus2-s2.0-85150338266en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1254en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2023.2185898
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3543
dc.identifier.volume53en_US
dc.identifier.wosWOS:000943894400001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDesirability function-embedded face-centered optimizationen_US
dc.subjectbioreductionen_US
dc.subject(S)-1-(thiophen-2-yl)ethanolen_US
dc.subjectWhole-cell biocatalystsen_US
dc.subjectWeissella cibaria N9en_US
dc.titleOptimization of asymmetric bioreduction conditions of 1-(thiophen-2-yl)ethanone by Weissella cibaria N9 using a desirability function-embedded face-centered optimization modelen_US
dc.typeArticleen_US

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