Recruiting grape-isolated antagonistic yeasts for the sustainable bio-management of Botrytis cinerea on grapes

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dc.authoridKarbancioglu Guler, Funda/0000-0001-6576-0084
dc.authoridOztekin, Sebahat/0000-0003-0859-1776
dc.contributor.authorOztekin, Sebahat
dc.contributor.authorKarbancioglu-Guler, Funda
dc.date.accessioned2024-10-04T18:52:46Z
dc.date.available2024-10-04T18:52:46Z
dc.date.issued2024
dc.departmentBayburt Üniversitesien_US
dc.description.abstractBotrytis cinerea is the causative agent of grey mould disease in grapes, which was linked to significant postharvest losses. This study examined three grape-isolated yeasts (Metschnikowia aff. fructicola, Metschnikowia pulcherrima, and Hansenispora uvarum) through in vitro and in vivo tests on detached grape berries against grey mould, as well as the elucidation of their possible mechanisms of action. The antifungal mechanism of action of yeasts was determined by the lytic enzyme activity, inhibition of spore germination, biofilm activity, iron depletion, diffusible metabolites, wound-site colonisation, mycocin, and volatile organic compounds (VOCs) production. The highest in vitro efficacy (83.13%) was observed on M. aff. fructicola, followed by M. pulcherrima (82.10%) and H. uvarum (71.66%). Metschnikowia yeasts exhibited comparable enzyme activities, including protease, beta-1,3 glucanase, gelatinase chitinase, and cellulase, while H. uvarum had a poor enzymatic activity with chitinase and gelatinase. M. aff. fructicola showed relatively higher iron depletion activity than M. pulcherrima, while M. pulcherrima outperformed via diffusible metabolites. All yeast cultures significantly reduced spore germination by at least 86%. Overall, M. aff. fructicola exhibited the highest biocontrol activity with its iron depletion, inhibition of conidial germination, biofilm formation, VOCs, and well colonisation on grape berries. M. aff. fructicola 1-UDM outperformed all other yeasts by significantly reducing disease incidence and lesion diameter values (93.4% and 94.3%, respectively). Remarkably, H. uvarum VOCs demonstrated potential as a biofumigant for suppressing grey mould. All yeasts are well adapted to their ecological niche to bio-protect grapes from grey mould disease.en_US
dc.description.sponsorshipThis work was financially supported by the Scientific Research Council of Istanbul Technical University under the grant number of MDK-2018-40878. In addition, the authors would like to thank the Wiley-TBIdot;TAK open access publishing agreement, whien_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1002/fes3.528
dc.identifier.issn2048-3694
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85184907016en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1002/fes3.528
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3645
dc.identifier.volume13en_US
dc.identifier.wosWOS:001159988900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofFood and Energy Securityen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectantagonistic yeastsen_US
dc.subjectbiological controlen_US
dc.subjectBotrytis cinereaen_US
dc.subjectgrapesen_US
dc.subjectgrey moulden_US
dc.subjectpostharvesten_US
dc.titleRecruiting grape-isolated antagonistic yeasts for the sustainable bio-management of Botrytis cinerea on grapesen_US
dc.typeArticleen_US

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